anti-IL-38, mAb (Nhat-1)
Method: Recombinant mouse IL-38 (AG-40B-0101) (lane 1), cell extracts from HEK 293 cells mock-transfected (lane 2) or transfected with human IL-38 (lane 3) were separated by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with anti-IL-38, mAb (Nhat-1) (1µg/ml). Proteins are visualized by a chemiluminescence detection system.
|Synonyms||Interleukin-38; Interleukin-1 Family Member 10; IL-1F10|
|Product Type||Monoclonal Antibody|
|Source/Host||Purified from concentrated hybridoma tissue culture supernatant.|
|Immunogen/Antigen||Recombinant human IL-38.|
Western Blot: (1:1'000)
Recognizes human and mouse IL-38.
|Purity Detail||Protein A affinity purified.|
|Formulation||Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.|
|Isotype Negative Control|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
|Use/Stability||Stable for at least 1 year after receipt when stored at -20°C.|
|Product Specification Sheet|
IL-38 (IL-1F10) belongs to the IL-1 family of proteins. IL-38 is expressed in heart, placenta, fetal liver, spleen, thymus and tonsil. The expression in a variety of immune tissues and similarity to IL-1Ra suggest a role of IL-38 as a IL-1 family receptor antagonist. Il-38 inhibits IL-36 signaling by binding to IL-1R6. IL-38 (aa 20-152) is released from apoptotic cells to limit inflammatory macrophage and downstream Th17 activation by blocking IL1RAPL1 (Interleukin-1 receptor accessory protein-like 1, TIGIRR-2) signaling. IL-38 is unregulated during some autoimmune diseases such as Systemic Lupus Erythematosus.