anti-Fibronectin (EDA), mAb (blocking) (IST-9) (preservative free)
AG-20B-6001PF-C100100 µgCHF 280.00
Immunohistochemical analysis on paraffin-embedded sections of human melanoma SK-MEL-28 using anti-Fibronectin (EDA), mAb (blocking) (IST-9) (preservative free) (AG-20B-6001PF).
Immunocytochemical analysis of human esophageal epithelial cells using anti-Fibronectin (EDA), mAb (blocking) (IST-9) (preservative free) (AG-20B-6001PF). An anti-mouse IgG secondary antibody was used. Nuclei were counterstained with propidium iodide.
Western blot analysis of human dermal fibroblast whole cell lysate, untreated (left) and treated with TGF-β1 (right) using anti-Fibronectin (EDA), mAb (blocking) (IST-9) (preservative free) (AG-20B-6001PF). Lysates/proteins at 10 µg per lane. A goat anti-mouse HRP-conjugated monoclonal secondary antibody was used.
|Synonyms||FN; Cold-insoluble Globulin; CIG; Fibronectin (EIIIA)|
|Product Type||Monoclonal Antibody|
|Source/Host||Purified from concentrated hybridoma tissue culture supernatant.|
|Immunogen/Antigen||Recombinant Extra Domain A (EDA) region of human cellular fibronectin.|
Functional Application: Inhibits binding of Fibronectin EDA domain to α5-β1, α4-β1 and α9-β1 integrins. Inhibits cell adhesion mediated by α5-β1, α4-β1 and α9-β1 integrins. Blocks the differentiation of fibroblasts into myo-fibroblasts and the TGF-β1-triggered enhancement of α-smooth muscle actin and collagen type I.
Immunohistochemistry: (frozen sections: 1-2μg/ml and paraffin sections: 2-10μg/ml)
Western Blot: 1:1000
|Specificity||Recognizes an epitope located in the EDA sequence of human cellular fibronectin (FN). Does not detect extracellular FN (plasma FN). Cross-reacts with mouse, rat, monkey, pig, dog, cow and chicken fibronectin EDA (according to literature).|
|Purity Detail||Epitope affinity purifed on recombinant fragment of fibronectin EDA.|
|Endotoxin Content||<0.001EU/μg purified protein (LAL test; Lonza).|
|Formulation||Liquid. In 20mM sodium phosphate buffer, pH7.6 containing 150mM sodium chloride.|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
|Use/Stability||Stable for at least 1 year after receipt when stored at -20°C.|
|Product Specification Sheet|
Fibronectin is a major component of the extracellular matrix that binds to integrins, cell surfaces and various compounds including collagen, fibrin, heparin, DNA and actin. It occurs in two main forms: plasma and cellular fibronectin. EDA (Extra Domain A)-containing cellular fibronectin (EDA-FN) plays a major role in cell adhesion, growth, migration and differentiation and it is important for processes such as wound healing and embryonic development. It is associated with fibroblast differentiation by increasing α-SMA expression, collagen deposition, cell contractility and focal adhesion kinase (FAK) activation. EDA-FN promotes cell spread by interacting with the integrins α5-β1, α4-β1 and α9-β1 and has been shown to promote angiogenesis, lymphangiogenesis and metastasis of colorectal tumors. In transformed or tumor-derived cells the EDA segment is about 10-times higher than in fibronectin from normal fibroblasts and it may therefore be a significant marker for malignancy.
- Monoclonal antibodies in the analysis of fibronectin isoforms generated by alternative splicing of mRNA precursors in normal and transformed human cells: L. Borsi, et al.; J. Cell Biol. 104, 595 (1987)
- Localization of the cellular-fibronectin-specific epitope recognized by the monoclonal antibody IST-9 using fusion proteins expressed in E. coli: B. Carnemolla, et al.; FEBS Lett. 215, 269 (1987)
- Molecular and immunologic differences in canine fibronectins from articular cartilage and plasma: N. Burton-Wurster & G. Lust; Arch. Biochem. Biophys. 269, 32 (1989)
- Coordinate oncodevelopmental modulation of alternative splicing of fibronectin pre-messenger RNA at ED-A, ED-B, and CS1 regions in human liver tumors: F. Oyama, et al.; Cancer Res. 53, 2005 (1993)
- Endogenous fibronectin of blood polymorphonuclear leukocytes: immunochemical characterization and subcellular localization: R. Salcedo, et al.; Exp. Cell Res. 233, 25 (1997)
- The fibronectin domain ED-A is crucial for myofibroblastic phenotype induction by transforming growth factor-beta1: G. Serini, et al.; J. Cell Biol. 142, 873 (1998)
- Identification of two amino acids within the EIIIA (ED-A) segment of fibronectin constituting the epitope for two function-blocking monoclonal antibodies: Y.F. Liao, et al.; J. Biol. Chem. 274, 17876 (1999)
- Engagement of alpha4beta7 integrins by monoclonal antibodies or ligands enhances survival of human eosinophils in vitro: J. Meerschaert, et al.; J. Immunol. 163, 6217 (1999)
- Segmental antigen challenge increases fibronectin in bronchoalveolar lavage fluid: J. Meerschaert, et al.; Am. J. Respir. Crit. Care Med. 159, 619 (1999)
- Deletion of the alternatively spliced fibronectin EIIIA domain in mice reduces atherosclerosis: M.H. Tan, et al.; Blood 104, 11 (2004)
- Fibronectin-alpha4beta1 integrin interactions regulate metalloproteinase-9 expression in steatotic liver ischemia and reperfusion injury: C. Moore, et al.; Am. J. Pathol. 170, 567 (2007)
- Identification of the peptide sequences within the EIIIA (EDA) segment of fibronectin that mediate integrin alpha9beta1-dependent cellular activities: A.V. Shinde, et al.; J. Biol. Chem. 283, 2858 (2008)
- Fibronectin extra domain A (EDA) sustains CD133+/CD44+ subpopulation of colorectal cancer cells: J. Ou, et al.; Stem Cell Res. 11, 820 (2013)
- Irigenin, a novel lead from Western Himalayan chemiome inhibits Fibronectin-Extra Domain A induced metastasis in lung cancer cells: A. Amin, et al.; Sci. Rep. 6, 37151 (2016)
- Detection of soluble ED-A+ fibronectin and evaluation as novel serum biomarker for cardiac tissue remodeling: B. Ziffels, et al.; Dis. Mark. 2016, ID3695454 (2016)