anti-Ubiquitin K48 Linkage, mAb (1001c)
AG-20T-0209-C05050 µgCHF 388.00
Western blot analysis using anti-Ubiquitin K48 Linkage, mAb (1001c) (Prod. No. AG-20T-0209). Method: 25ng of each linkage of recombinant di-ubiquitin was run on a 10-20% SDS-PAGE gel prior to blotting on PVDF membrane. Western blots were developed using anti-Ubiquitin K48 Linkage, mAb (1001c) or anti-Ubiquitin (human), mAb (83406) (Prod. No. AG-20T-0208) primary antibodies at 0.5 μg/ml. The appropriate HRP-labeled anti-rabbit or anti-mouse secondary antibodies were used at a 1:2000 dilution. A single band of appropriate size was detected only in the K48-linked diubiquitin lane using anti-Ubiquitin K48 Linkage, mAb (1001c).
Western blot analysis using anti-Ubiquitin K48 Linkage, mAb (1001c) (Prod. No. AG-20T-0209). Method: 20μg of cellular protein extracts from HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) for 4 hours with 10μM MG132 were run on a reducing SDS-PAGE gel. Proteins were blotted to PVDF prior to analysis with anti-Ubiquitin K48 Linkage, mAb (1001c) primary antibody at 0.05 μg/ml followed by HRP-labeled anti-rabbit secondary antibody at 1:2000 dilution. As expected MG132 (a proteasome inhibitor) treatment resulted in the accumulation of K48-linked polyubiquitin chains attached to cellular proteins as detected by anti-Ubiquitin K48 Linkage, mAb (1001c).
|Synonyms||UBB; Ubiquitin B|
|Product Type||Monoclonal Antibody|
|Immunogen/Antigen||K48-linkage from human ubiquitin.|
Western Blot: (0.1-0.5µg/ml)
|Specificity||Recognizes endogenous human proteins containing K48-linked polyubiquitin chains, but has no cross-reactivity to monoubiquitin or polyubiquitin of other linkages.|
|Purity Detail||Protein A purified.|
|Formulation||Liquid. In PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.|
|Declaration||Manufactured by Boston Biochem|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
|Handling Advice||Aliquot to avoid freeze/thaw cycles.|
|Use/Stability||Stable for at least 1 year after receipt when stored at -20°C.|
|Product Specification Sheet|
Polyubiquitin chains are composed of ubiquitin monomers that are covalently linked through isopeptide bonds (other than linear, or “Met1-linked” polyubiquitin). Isopeptides are formed between a lysine residue of one ubiquitin molecule and the C-terminal glycine residue of another ubiquitin molecule. Seven of the seventy-six amino acids in ubiquitin are lysine residues that can participate in polyubiquitin chain formation. Linkage through specific lysine residues is thought to serve as a signal that affects protein degradation, signaling, trafficking, and other cellular processes. K48-linked polyubiquitin chains attached to substrate proteins often serve as a recognition sequence for targeting and destruction of the substrate by the 26S proteasome.