anti-Leptin (mouse), pAb (Biotin)

CHF 390.00
In stock
AG-25A-0008B-C05050 µgCHF 390.00
 
More Information
Product Details
Synonyms OB Gene Product; Obese Factor
Product Type Polyclonal Antibody
Properties
Source/Host Rabbit
Immunogen/Antigen Recombinant mouse leptin.
Label/Conjugates Biotin
Application ELISA: (direct and indirect: 1:5’000-1:10’000)
Immunoprecipitation: (1-5 μl per sample in a final reaction volume of 0.5-1 ml)
Western Blot: (1:5’000-1:10’000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.
Crossreactivity Mouse
Specificity Recognizes mouse leptin. Weakly cross-reacts with human and rat leptin. Detects a band of ~16kDa by Western blot.
Purity Detail Protein A-affinity purified.
Concentration 1mg/ml
Formulation Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Leptin is involved in the signaling pathway that regulates the size of the body fat depot. An increase in the level of leptin may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Defects in the leptin gene may be a cause of autosomal recessive obesity.
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