CD137L, Soluble (mouse) (rec.)
HEK 293 cells were transiently transfected with a construct encoding full-length mouse CD137. At 48hrs after transfection, the transfectants were incubated with 0.5 or 5μg/ml CD137L, Soluble (mouse) (rec.) (Prod. No. AG-40A-0020Y) and followed by flow cytometry analysis with anti-FLAG® M2-FITC.
|Source/Host||HEK 293 cells|
|Sequence||The extracellular domain of mouse CD137L (aa 104-309) is fused at the N-terminus to a FLAG®-tag.|
|Specificity||Binds to mouse CD137.|
|Endotoxin Content||<0.01EU/μg purified protein (LAL test; Lonza).|
|Reconstitution||Reconstitute with 50μl sterile water.|
|Formulation||Lyophilized. Contains PBS.|
|Other Product Data||
UniProt link P41274: CD137L (mouse)
FLAG is a registered trademark of Sigma-Aldrich Co.
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
|Product Specification Sheet|
The CD137 ligand (CD137L; 4-1BBL) is a member of the tumor necrosis factor (TNF) family. CD137L is a type II, transmembrane protein found on activated macrophages, dendritic cells and mature B cells. The interaction with its receptor 4-1BB induces recruitment of TNF receptor-associated factor 1 (TRAF1) and TRAF2 and interaction with the kinase p56lck. CD137 and CD137L have been reported to be involved in tumor rejection, apoptosis, anti-viral immunity, diabetes, in T and B cell co-stimulation and modulation of the immune response.