Slit2 (C fragment) (human) (rec.)
|Synonyms||Slit Homolog 2 Protein (C fragment)|
|Source/Host||HEK 293 cells|
|Sequence||Human Slit2 (aa 1122-1529) is fused at the C-terminus to a FLAG®-tag.|
|Biological Activity||Activates an UCP1-luciferase construct in mouse fat primary cells at a concentration of 20nM.|
|Endotoxin Content||<0.01EU/μg purified protein (LAL test; Lonza).|
|Concentration||0.1mg/ml after reconstitution.|
|Reconstitution||Reconstitute with 100μl sterile water.|
|Formulation||Lyophilized. Contains PBS.|
|Other Product Data||Uni-Prot link 0904813: Slit2 (C fragment)|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.
Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
|Product Specification Sheet|
The Slits are secreted proteins that bind to Robo receptors and play a role in axon guidance and neuronal migration. The Slit family in mice and humans comprises three members: Slit1, Slit2 and Slit3. These are all extracellular matrix proteins of 180-200kDa. In vivo, Slit2 is cleaved into 140kDa N-terminal (Slit2-N) and 55-60 kDa C-terminal (Slit2-C) fragments. Only Slit2-N and Slit2 full-length bind the Robo proteins. Recently, it has been shown that Slit2 is highly expressed in adipose tissues under the control of PRDM16 and cold exposure. Slit2 is posttranslationally cleaved by adipocytes and the resulting Slit2-C fragment improves glucose homeostasis in mice by activating a thermogenic PKA/cAMP pathway not mediated by β-adrenergic receptors in adipocytes.