|Product Type||Polyclonal Antibody|
|Application||FACS, ICC, IHC, IM, WB|
|Formulation||Liquid. In PBS, 0.02% sodium azide.|
|Other Product Data||
Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.
|Declaration||Manufactured by BioActs.|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
Keep cool and dry.
Protect from light.
|Use/Stability||Stable for at least 1 year after receipt when stored at -20°C.|
|Product Specification Sheet|
Autophagy is the digesting phenomenon, including cell apparatus, one self’s metabolite and part of the cytoplasm. In Macroautophage, one of the Autophagy, first, there appears isolation membrane structure and forms Autophagosome which is about 1um diameter. Autophagy is double layered membranes and can be digested into closed cytosol in the inner membrane, combined with lysosome which have digestive enzyme. LC3 Protein which is conjugated protein of Autophagy can be useful as a marker for Autophagosome. You can use Goat anti-Rabbit IgG combined Flamma® series which is various kinds of fluorescent pigments with horseradish peroxidase (HRP) along with LC3 Protein for Autophagosome-specific marker among cell organelle. Dilution Flow Cytometry (Flow) 1:200, Immunocytochemistry (ICC) 0.1-2 µg/ml, Immunofluorescence (IF) 0.1-2 µg/ml, Immunohistochemistry (Frozen) (IHC (F)) 1:400, Immunohistochemistry (Paraffin) (IHC (P)) 1:200-1:400, Immunomicroscopy (IM) Assay-Dependent, Western Blot (WB) 1:1000.