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CX3CL1, Soluble (human) (rec.)
210
CHF
CHF 210.00
In stock
CHI-AG-40A-0079-C01010 µgCHF 210.00
CHI-AG-40A-0079-C05050 µgCHF 600.00
Replaces Prod. No. AG-40A-0079
| Product Details | |
|---|---|
| Synonyms | Fractalkine; Chemokine (C-X3-C Motif) Ligand 1; Neurotactin; SCYD1 |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells |
| Sequence |
The extracellular domain of human CX3CL1 (aa 25-339) is fused at the C-terminus to a FLAG®-tag. |
| Crossreactivity | Human |
| MW | ~50kDa (SDS-PAGE) |
| Purity | ≥90% (SDS-PAGE) |
| Endotoxin Content | <0.06EU/μg purified protein (LAL test). |
| Reconstitution |
Reconstitute 10µg vial with 100 µl sterile water to a concentration of 0.1mg/ml. Reconstitute 50µg vial with 100 µl sterile water to a concentration of 0.5mg/ml. Add 1X PBS to the desired protein concentration. |
| Accession Number | AAH01163.1 |
| Formulation | Lyophilized from 0.2μm-filtered solution in PBS. |
| Other Product Data |
UniProt link AAH01163.1: CX3CL1 (Fractalkine) (human) FLAG is a registered trademark of Sigma-Aldrich Co. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
CX3CL1 [Fractalkine] is expressed on TNF-α and IL-1 activated endothelial cells (ECs) and binds to CX3CR1. CX3CL1 can exist in 2 forms, as membrane-anchored or as a shed 95-kDa glycoprotein. The soluble CX3CL1 has potent chemoattractant activity for T cells and monocytes, and the cell surface bound protein, which is induced on activated primary endothelial cells, promotes strong adhesion of those leukocytes. CX3CL1 may play a role in regulating leukocyte adhesion and migration processes at the endothelium.
Product References
- Role of Fractalkine in promoting inflammation in sepsis-induced multiple organ dysfunction: X. Chen, et al.; Inf. Gen. Evol. 85, 104569 (2020)
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