CX3CL1, Soluble (human) (rec.)

CHF 200.00
In stock
CHI-AG-40A-0079-C01010 µgCHF 200.00
CHI-AG-40A-0079-C05050 µgCHF 590.00
Replaces Prod. No. AG-40A-0079
More Information
Product Details
Synonyms Fractalkine; Chemokine (C-X3-C Motif) Ligand 1; Neurotactin; SCYD1
Product Type Protein
Properties
Source/Host HEK 293 cells
Sequence

The extracellular domain of human CX3CL1 (aa 25-339) is fused at the C-terminus to a FLAG®-tag.

Crossreactivity Human
MW ~50kDa (SDS-PAGE)
Purity ≥90% (SDS-PAGE)
Endotoxin Content <0.06EU/μg purified protein (LAL test).
Reconstitution Reconstitute 10µg vial with 100 µl sterile water to a concentration of 0.1mg/ml.
Reconstitute 50µg vial with 100 µl sterile water to a concentration of 0.5mg/ml.
Add 1X PBS to the desired protein concentration.
Accession Number AAH01163.1
Formulation Lyophilized from 0.2μm-filtered solution in PBS.
Other Product Data

UniProt link AAH01163.1: CX3CL1 (Fractalkine) (human)

FLAG is a registered trademark of Sigma-Aldrich Co.

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

CX3CL1 [Fractalkine] is expressed on TNF-α and IL-1 activated endothelial cells (ECs) and binds to CX3CR1. CX3CL1 can exist in 2 forms, as membrane-anchored or as a shed 95-kDa glycoprotein. The soluble CX3CL1 has potent chemoattractant activity for T cells and monocytes, and the cell surface bound protein, which is induced on activated primary endothelial cells, promotes strong adhesion of those leukocytes. CX3CL1 may play a role in regulating leukocyte adhesion and migration processes at the endothelium.

Product References
  1. Role of Fractalkine in promoting inflammation in sepsis-induced multiple organ dysfunction: X. Chen, et al.; Inf. Gen. Evol. 85, 104569 (2020)
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