CX3CL1, Soluble (human) (rec.)
|Synonyms||Fractalkine; Chemokine (C-X3-C Motif) Ligand 1; Neurotactin; SCYD1|
|Source/Host||HEK 293 cells|
The extracellular domain of human CX3CL1 (aa 25-339) is fused at the C-terminus to a FLAG®-tag.
|Endotoxin Content||<0.06EU/μg purified protein (LAL test).|
Reconstitute 10µg vial with 100 µl sterile water to a concentration of 0.1mg/ml.
Reconstitute 50µg vial with 100 µl sterile water to a concentration of 0.5mg/ml.
Add 1X PBS to the desired protein concentration.
|Formulation||Lyophilized from 0.2μm-filtered solution in PBS.|
|Other Product Data||
UniProt link AAH01163.1: CX3CL1 (Fractalkine) (human)
FLAG is a registered trademark of Sigma-Aldrich Co.
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
|Product Specification Sheet|
CX3CL1 [Fractalkine] is expressed on TNF-α and IL-1 activated endothelial cells (ECs) and binds to CX3CR1. CX3CL1 can exist in 2 forms, as membrane-anchored or as a shed 95-kDa glycoprotein. The soluble CX3CL1 has potent chemoattractant activity for T cells and monocytes, and the cell surface bound protein, which is induced on activated primary endothelial cells, promotes strong adhesion of those leukocytes. CX3CL1 may play a role in regulating leukocyte adhesion and migration processes at the endothelium.
- Role of Fractalkine in promoting inflammation in sepsis-induced multiple organ dysfunction: X. Chen, et al.; Inf. Gen. Evol. 85, 104569 (2020)