IL-3 (human) (rec.) (His)

CHF 240.00
In stock
CHI-HF-20103-C01010 µgCHF 240.00
CHI-HF-20103-C05050 µgCHF 710.00
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Product Details
Synonyms Interleukin-3
Product Type Protein
Properties
Source/Host HEK 293 cells
Sequence Human IL-3 (aa 20-152) is fused at the C-terminus to a His-tag.
Crossreactivity Human
Biological Activity Measured by its ability to stimulate the proliferation of human TF-1 cells. The ED50 for this effect is typically 0.1ng/ml, corresponding to a specific activity of 1x 107 units/mg
MW ~20kDa (SDS-PAGE)
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.01EU/μg protein (LAL test; Lonza).
Reconstitution Reconstitute 10µg vial with 100 µl sterile water to a concentration of 0.1mg/ml.
Reconstitute 50µg vial with 100 µl sterile water to a concentration of 0.5mg/ml.
Add 1X PBS to the desired protein concentration.
Formulation Lyophilized from 0.2μm-filtered solution in PBS.
Other Product Data NCBI reference AAH69472.1: IL-3 (human)
Declaration Manufactured by Chimerigen.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
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Product Specification Sheet
Datasheet Download PDF

IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoiesis, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is a species-specific, variably glycosylated cytokine. Recombinant Human IL-3 is a 15.0 kDa globular protein containing 133 amino acid residues.

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