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SCF (human) (rec.) (His)
250
CHF
CHF 250.00
In stock
CHI-HF-201SCF-C01010 µgCHF 250.00
CHI-HF-201SCF-C05050 µgCHF 720.00
Product Details | |
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Synonyms | Stem Cell Factor; c-Kit Ligand; Kit Ligand; Mast Cell Grow Factor; MGF; SHEP7; FPHH |
Product Type | Protein |
Properties | |
Source/Host | HEK 293 cells |
Sequence | Human SCF (aa 26-187) is fused at the C-terminus to a His-tag. |
Crossreactivity | Human |
Biological Activity | Measured by its ability to stimulate the proliferation of human TF-1 cells. The ED50 for this effect is typically 5.0ng/ml, corresponding to a specific activity of 2x 105 units/mg |
MW | ~23kDa (SDS-PAGE) |
Purity | ≥95% (SDS-PAGE) |
Endotoxin Content | <0.01EU/μg protein (LAL test; Lonza). |
Reconstitution |
Reconstitute 10µg vial with 100 µl sterile water to a concentration of 0.1mg/ml. Reconstitute 50µg vial with 100 µl sterile water to a concentration of 0.5mg/ml. Add 1X PBS to the desired protein concentration. |
Formulation | Lyophilized from 0.2μm-filtered solution in PBS. |
Other Product Data | NCBI reference NP_003985.2: SCF (human) |
Declaration | Manufactured by Chimerigen. |
Shipping and Handling | |
Shipping | BLUE ICE |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice |
Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
Use/Stability |
Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
Documents | |
MSDS | Inquire |
Product Specification Sheet | |
Datasheet |
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Description
Stem cell factor (SCF), also known as cKit ligand (KL), mast cell growth factor (MGF) and steel factor (SLF), is a widely expressed 28-40 kDa type I transmembrane glycoprotein. It promotes the survival, differentiation and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell and melanocyte progenitors. SCF is a primary growth and activation factor for mast cells and eosinophils. Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/cKit to trigger receptor dimerization and signaling. SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels.