SCF (human) (rec.) (His)

CHF 240.00
In stock
CHI-HF-201SCF-C01010 µgCHF 240.00
CHI-HF-201SCF-C05050 µgCHF 710.00
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Product Details
Synonyms Stem Cell Factor; c-Kit Ligand; Kit Ligand; Mast Cell Grow Factor; MGF; SHEP7; FPHH
Product Type Protein
Properties
Source/Host HEK 293 cells
Sequence Human SCF (aa 26-187) is fused at the C-terminus to a His-tag.
Crossreactivity Human
Biological Activity Measured by its ability to stimulate the proliferation of human TF-1 cells. The ED50 for this effect is typically 5.0ng/ml, corresponding to a specific activity of 2x 105 units/mg
MW ~23kDa (SDS-PAGE)
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.01EU/μg protein (LAL test; Lonza).
Reconstitution Reconstitute 10µg vial with 100 µl sterile water to a concentration of 0.1mg/ml.
Reconstitute 50µg vial with 100 µl sterile water to a concentration of 0.5mg/ml.
Add 1X PBS to the desired protein concentration.
Formulation Lyophilized from 0.2μm-filtered solution in PBS.
Other Product Data NCBI reference NP_003985.2: SCF (human)
Declaration Manufactured by Chimerigen.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF

Stem cell factor (SCF), also known as cKit ligand (KL), mast cell growth factor (MGF) and steel factor (SLF), is a widely expressed 28-40 kDa type I transmembrane glycoprotein. It promotes the survival, differentiation and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell and melanocyte progenitors. SCF is a primary growth and activation factor for mast cells and eosinophils. Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/cKit to trigger receptor dimerization and signaling. SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels.

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