IL-22 (human):Fc (human) (rec.) (non-lytic)

CHF 320.00
In stock
CHI-HF-22022-C02525 µgCHF 320.00
CHI-HF-22022-C05050 µgCHF 460.00
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Product Details
Synonyms Interleukin-22; IL-10-related T Cell-derived-inducible Factor
Product Type Protein
Source/Host CHO cells

The extracellular domain of human IL-22 (aa 34-179) is fused to the N-terminus of the Fc region of a mutant human IgG1.

Crossreactivity Human
Biological Activity

Shows the biological function of the IL-22 moiety and exerts a prolonged circulating half-life caused by the modified Fc domain.

MW ~46kDa
Purity ≥95% (SDS-PAGE)
Endotoxin Content <1EU/mg protein (LAL test; Lonza).
Reconstitution Reconstitute 25µg vial in 250µl sterile water.
Reconstitute 50µg vial in 50µl sterile water.
Add 1X PBS to the desired protein concentration.
Formulation Lyophilized from 0.2μm-filtered solution in PBS.
Protein Negative Control

Fc (human) IgG1 Control (rec.) (non-lytic)

Other Product Data

Non-lytic: Acts as a long lasting fusion protein which only binds to the receptor. Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).

NCBI reference NP_065386.1: IL-22 (human)

Declaration Manufactured by Chimerigen.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF

The IL-22 gene includes five exons and a 537 bp-long open reading frame that encodes for a 179 amino acid protein. IL-22 is a member of the interleukin-10 (IL-10) family of cytokines which includes nine members, IL-10; the IL-20 subfamily members IL-19, IL-20, IL-22, IL-24, and IL-26; and the distantly related cytokines IL-28A, IL-28B, and IL-29, which are more commonly classified as type III interferons (IFNs) and designated as IFN-λ2, IFN-λ3, and IFN-λ1. Mouse and human IL-22 share 79% homology.  IL-22 is mainly produced by lymphocytes, such as T helper type 1 (Th1), Th17, Th22, CD8+ T cells, γδT cells, natural killer cells, lymphoid tissue inducer cells, innate lymphoid type 3 (ILC3) cells, and also by neutrophils. IL-22 has two heterodimeric transmembrane receptors, IL-22R1 and IL-10R2, which subsequently activate the JAK/STAT3, ERK and JNK pathways.

IL-22 responsiveness is limited by epithelial cell-restricted expression of IL-22RA1 in the lung, gastrointestinal tract, thymus, skin, pancreas, liver and kidney and represents a major communication channel between the immune system and specialized tissue cell types. IL-22 is a critical regulator of epithelial homeostasis, implicated in multiple aspects of epithelial barrier function, including regulation of epithelial cell growth and permeability, production of mucus and antimicrobial proteins (AMPs) and complement production. IL-22 plays key functions in the intestine and, therefore, plays a protective role in inflammatory bowel disease (IBD). IL-22 is also a controversial cytokine in tumor development; the IL-22-STAT3 axis induces anti-apoptotic genes and provides survival and proliferation signals for both normal and malignant cells. Therefore, in healthy conditions, it prevents tumor formation; however, once a tumor has been established, IL-22 promotes tumorigenesis.

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