GM-CSF (human) (rec.) (His)

CHF 140.00
In stock
CHI-HR-200CSF-C01010 µgCHF 140.00
CHI-HR-200CSF-C05050 µgCHF 440.00
 
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Product Details
Synonyms CSF; CSF2; GMCSF; GM-CSF; Molgramostin; Sargramostim; Colony-stimulating Factor; Granulocyte-macrophage Colony-stimulating Factor
Product Type Protein
Properties
Source/Host E. coli
Sequence Human GM-CSF (aa 18-144) is fused at the C-terminus to a His-tag.
Crossreactivity Human
Biological Activity Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.6 ng/mL.
MW ~16kDa (SDS-PAGE)
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.1EU/µg protein (LAL test; Lonza).
Reconstitution Reconstitute 10µg vial with 100 µl sterile water to a concentration of 0.1mg/ml.
Reconstitute 50µg vial with 100 µl sterile water to a concentration of 0.5mg/ml.
Add 1X PBS to the desired protein concentration.
Formulation Lyophilized from 0.2μm-filtered solution in 50mM Tris-HCl, 200mM NaCl, pH8.0.
Other Product Data NCBI reference AAI14000.1: GM-CSF (human)
Declaration Manufactured by Chimerigen.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF
Human GM-CSF is a 24 kDa glycoprotein produced by a variety of cell types, including T and B lymphocytes, macrophages, keratinocytes, eosinophils, neutrophils, and endothelial cells in response to cytokine or inflammatory stimuli. GM-CSF was initially identified on the basis of its capacity to stimulate the clonal proliferation of myeloid precursors in vitro. GM-CSF promotes a Th1 biased immune response, allergic inflammation, and the development of autoimmunity. GM-CSF are particularly effective as antitumor vaccines. GM-CSF regulates multiple biological activities through activation of the GM-CSF receptor (GMCSFR), a member of the type I cytokine receptor group comprising α and β subunits.
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