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GM-CSF (human) (rec.) (His)
130
CHF
CHF 130.00
In stock
CHI-HR-200CSF-C01010 µgCHF 130.00
CHI-HR-200CSF-C05050 µgCHF 370.00
| Product Details | |
|---|---|
| Synonyms | CSF; CSF2; GMCSF; GM-CSF; Molgramostin; Sargramostim; Colony-stimulating Factor; Granulocyte-macrophage Colony-stimulating Factor |
| Product Type | Protein |
| Properties | |
| Source/Host | E. coli |
| Sequence | Human GM-CSF (aa 18-144) is fused at the C-terminus to a His-tag. |
| Crossreactivity | Human |
| Biological Activity | Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.6 ng/mL. |
| MW | ~16kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.1EU/µg protein (LAL test; Lonza). |
| Reconstitution |
Reconstitute 10µg vial with 100 µl sterile water to a concentration of 0.1mg/ml. Reconstitute 50µg vial with 100 µl sterile water to a concentration of 0.5mg/ml. Add 1X PBS to the desired protein concentration. |
| Formulation | Lyophilized from 0.2μm-filtered solution in 50mM Tris-HCl, 200mM NaCl, pH8.0. |
| Other Product Data | NCBI reference AAI14000.1: GM-CSF (human) |
| Declaration | Manufactured by Chimerigen. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Human GM-CSF is a 24 kDa glycoprotein produced by a variety of cell types, including T and B lymphocytes, macrophages, keratinocytes, eosinophils, neutrophils, and endothelial cells in response to cytokine or inflammatory stimuli. GM-CSF was initially identified on the basis of its capacity to stimulate the clonal proliferation of myeloid precursors in vitro. GM-CSF promotes a Th1 biased immune response, allergic inflammation, and the development of autoimmunity. GM-CSF are particularly effective as antitumor vaccines. GM-CSF regulates multiple biological activities through activation of the GM-CSF receptor (GMCSFR), a member of the type I cytokine receptor group comprising α and β subunits.
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