Cookie Policy: This site uses cookies to improve your experience. You can find out more about our use of cookies in our Privacy Policy. By continuing to browse this site you agree to our use of cookies.
FGF-2 (human) (rec.)
130
CHF
CHF 130.00
In stock
CHI-HR-200FGF2-C01010 µgCHF 130.00
CHI-HR-200FGF2-C05050 µgCHF 370.00
| Product Details | |
|---|---|
| Synonyms | Basic Fibroblast Growth Factor; bFGF; Heparin Binding Growth Factor-2; HBGF-2 |
| Product Type | Protein |
| Properties | |
| Source/Host | E. coli |
| Sequence | Human FGF-2 (aa 143-288). |
| Crossreactivity | Human |
| Biological Activity | Measured by its ability to stimulate the proliferation of balb/c3T3 cells. The ED50 for this effect is typically 0.1ng/ml, corresponding to a specific activity of 1x 107 units/mg. |
| MW | ~17kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.1EU/μg purified protein (LAL test; Lonza). |
| Reconstitution |
Reconstitute 10µg vial with 100 µl sterile water to a concentration of 0.1mg/ml. Reconstitute 50µg vial with 100 µl sterile water to a concentration of 0.5mg/ml. Add 1X PBS to the desired protein concentration. |
| Formulation | Lyophilized from 0.2µm-filtered solution in 50mM Tris-HCl, 400nM NaCl, pH8.0. |
| Other Product Data | NCBI reference NP_001997.5: FGF-2 (human) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
FGF-2 (Basic fibroblast growth factor) belongs to the FGF superfamily that is made up of 22 different fibroblast growth factor genes. FGF-2 and FGF-1 (acidic FGF) are unique in that they do not follow the conventional signal sequence for secretion. FGF-2 is found in five isoforms via alternative initiation of translation. Signaling of FGF-2 occurs through the high-affinity tyrosine kinase receptors FGFR1-4. FGF-2 is a potent wide-spectrum mitogen whose overexpression is associated with immortalization and unregulated cell proliferation in many tumors. FGF-2 functions in angiogenesis, cell survival, tissue repair, embryonic development and inflammation. FGF-2 is widely used to maintain the self-renewal of human embryonic stem cells (ESCs) and to induce pluripotent stem cells. FGF-2 is an established neurogenic factor for proliferation and differentiation of multipotent neural stem cells both during development and in the adult mouse brain as well.
Related Products
