FGF-2 (human) (rec.)

CHF 140.00
In stock
CHI-HR-200FGF2-C01010 µgCHF 140.00
CHI-HR-200FGF2-C05050 µgCHF 440.00
 
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Product Details
Synonyms Basic Fibroblast Growth Factor; bFGF; Heparin Binding Growth Factor-2; HBGF-2
Product Type Protein
Properties
Source/Host E. coli
Sequence Human FGF-2 (aa 143-288).
Crossreactivity Human
Biological Activity Measured by its ability to stimulate the proliferation of balb/c3T3 cells. The ED50 for this effect is typically 0.1ng/ml, corresponding to a specific activity of 1x 107 units/mg.
MW ~17kDa (SDS-PAGE)
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.1EU/μg purified protein (LAL test; Lonza).
Reconstitution Reconstitute 10µg vial with 100 µl sterile water to a concentration of 0.1mg/ml.
Reconstitute 50µg vial with 100 µl sterile water to a concentration of 0.5mg/ml.
Add 1X PBS to the desired protein concentration.
Formulation Lyophilized from 0.2µm-filtered solution in 50mM Tris-HCl, 400nM NaCl, pH8.0.
Other Product Data NCBI reference NP_001997.5: FGF-2 (human)
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF
FGF-2 (Basic fibroblast growth factor) belongs to the FGF superfamily that is made up of 22 different fibroblast growth factor genes. FGF-2 and FGF-1 (acidic FGF) are unique in that they do not follow the conventional signal sequence for secretion. FGF-2 is found in five isoforms via alternative initiation of translation. Signaling of FGF-2 occurs through the high-affinity tyrosine kinase receptors FGFR1-4. FGF-2 is a potent wide-spectrum mitogen whose overexpression is associated with immortalization and unregulated cell proliferation in many tumors. FGF-2 functions in angiogenesis, cell survival, tissue repair, embryonic development and inflammation. FGF-2 is widely used to maintain the self-renewal of human embryonic stem cells (ESCs) and to induce pluripotent stem cells. FGF-2 is an established neurogenic factor for proliferation and differentiation of multipotent neural stem cells both during development and in the adult mouse brain as well.
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