IL-10 (human) (rec.) (untagged)

CHF 260.00
In stock
CHI-HR-20610-C01010 µgCHF 260.00
CHI-HR-20610-C05050 µgCHF 760.00
More Information
Product Details
Synonyms Interleukin-10; Cytokine Synthesis Inhibitory Factor; CSIF
Product Type Protein
Properties
Source/Host E. coli
Sequence

Human IL-10 (aa 19-178) with a N-terminal Met.

Crossreactivity Human
Biological Activity

Immobilized human IL-10 at 10µg/ml (100µl/well) can bind Cynomolgus IL-10RA-Fc. The EC50 of Cynomolgus IL-10RA-Fc is 0.24-0.56µg/ml.

MW ~18kDa (SDS-PAGE)
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.01EU/μg protein (LAL test; Lonza).
Reconstitution Reconstitute 10µg vial with 100 µl sterile water to a concentration of 0.1mg/ml.
Reconstitute 50µg vial with 100 µl sterile water to a concentration of 0.5mg/ml.
Further dilute in other aqueous solutions.
Formulation Lyophilized from 0.2μm-filtered solution in 20mM Tris, 20mM NaCl, pH8.7.
Other Product Data

NCBI reference NP_000563.1: IL-10 (human)

Declaration Manufactured by Chimerigen.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -80°C
Handling Advice Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
Use/Stability Stable for at least 1 year after receipt when stored at -80°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF

Interleukin-10 (IL-10) is a cytokine produced by activated Th2 cells, B cells, keratinocytes and monocytes/macrophages. In vitro murine and human IL-10 inhibits cytokine synthesis by Th1 cells, natural killer cells and monocytes/macrophages. Several studies have suggested the potential application of IL-10 as an anti-inflammatory agent in the treatment of septic shock and as an immunosuppressive agent in certain T cell mediated autoimmune diseases.

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