IL-10 (human) (rec.) (untagged)
|Synonyms||Interleukin-10; Cytokine Synthesis Inhibitory Factor; CSIF|
Human IL-10 (aa 19-178) with a N-terminal Met.
Immobilized human IL-10 at 10µg/ml (100µl/well) can bind Cynomolgus IL-10RA-Fc. The EC50 of Cynomolgus IL-10RA-Fc is 0.24-0.56µg/ml.
|Endotoxin Content||<0.01EU/μg protein (LAL test; Lonza).|
Reconstitute 10µg vial with 100 µl sterile water to a concentration of 0.1mg/ml.
Reconstitute 50µg vial with 100 µl sterile water to a concentration of 0.5mg/ml.
Further dilute in other aqueous solutions.
|Formulation||Lyophilized from 0.2μm-filtered solution in 20mM Tris, 20mM NaCl, pH8.7.|
|Other Product Data||
NCBI reference NP_000563.1: IL-10 (human)
|Declaration||Manufactured by Chimerigen.|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-80°C|
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
Stable for at least 1 year after receipt when stored at -80°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
|Product Specification Sheet|
Interleukin-10 (IL-10) is a cytokine produced by activated Th2 cells, B cells, keratinocytes and monocytes/macrophages. In vitro murine and human IL-10 inhibits cytokine synthesis by Th1 cells, natural killer cells and monocytes/macrophages. Several studies have suggested the potential application of IL-10 as an anti-inflammatory agent in the treatment of septic shock and as an immunosuppressive agent in certain T cell mediated autoimmune diseases.