Polyinosinic-polycytidylic acid [Poly(I:C)] Endotoxin-free (sterile)
|Synonyms||Polyinosinic-polycytidylic acid, potassium salt (poly (I:C))|
|MW||High molecular weight (>1.5kb).|
|Reconstitution||Just before use, prepare a stock solution in endotoxin-free and sterile ddWater (Cat. No.: IAX-900-002), or PBS (Cat. No.: IAX-900-001).|
Activates TLR3, MDA5/Helicard and RIG-I. Optimal working concentration depends upon target receptor accessibility, number, density, as well as cell type, and therefore needs to be determined for each application. Recommended concentration range: in vitro: 0.5-10μg/ml; in vivo: 5mg/kg.
|Declaration||Manufactured by Innaxon.|
|Other Product Data||
Contains: 2mg and 5mg sizes include 1.5ml ddWater Endotoxin-free (sterile) (Cat. No.: IAX-900-002-LD15). 10mg size includes 10ml ddWater Endotoxin-free (sterile) (Cat. No.: IAX-900-002-L010).
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||+4°C|
Avoid freeze/thaw cycles.
After reconstitution, prepare aliquots and keep aqueous stock solutions for 1 day at 4°C or store at -20°C (shelf-life 6 months).
|Use/Stability||Stable for at least 2 years after receipt when stored at +4°C.|
|Product Specification Sheet|
Toll-like receptor (TLR) 3 is an endosomal TLR that mediates immune responses against viral infections upon activation by its ligand double-stranded RNA, a replication intermediate of most viruses. TLR3 is expressed widely in the body and activates both the innate and adaptive immune systems. Toll-like receptor 3 (TLR3), melanoma differentiation-associated gene 5 (MDA5), and retinoic acid-inducible gene-I (RIG-I), all sensors of double-stranded RNA (dsRNA) are potent inducers of antiviral activity. dsRNA sensor activation -e.g. by poly (I:C)- induces pro-inflammatory TNF-α and antiviral IFN-β, but can also enhance the expression of pro-apoptotic proteins. Recently, poly (I:C)-induced cell death recently gained considerable attention as a tool to study the 'Ripoptosome' or 'Necrosome' complex, a novel intracellular signaling complex, thought to induce regulated necrosis, also called 'Necroptosis'.
- The 'complexities' of life and death: Death receptor signalling platforms: L.S. Dickens, et al. Exp. Cell Res. 18, 1269 (2012)
- Inhibitor of apoptosis proteins limit RIP3 kinase-dependent interleukin-1 activation: J.E. Vince, et al. Immunity 36, 215 (2012)
- 'Necrosome'-induced inflammation: must cells die for it? D. Wallach, et al.; Trends Immunol. 32, 505 (2011)
- Pick your poison: the Ripoptosome, a cell death platform regulating apoptosis and necroptosis: M. Feoktistova, et al.; Cell Cycle 11, 460 (2012)
- Ripoptosome: a novel IAP-regulated cell death-signalling platform: G. Imre, et al.; Mol. Cell Biol. 3, 324 (2011)
- IAPs: guardians of RIPK1: M. Darding & P. Meier; Cell Death Differ. 19, 58 (2012)
- The Ripoptosome: death decision in the cytosol: M.J. Bertrand & P. Vandenabeele; Mol. Cell 43, 323 (2011)
- cIAPs block Ripoptosome formation, a RIP1/caspase-8 containing intracellular cell death complex differentially regulated by cFLIP isoforms: M. Feoktistova, et al.; Mol. Cell 43, 449 (2011)
- The Ripoptosome, a signaling platform that assembles in response to genotoxic stress and loss of IAPs: T. Tenev, et al.; Mol. Cell 43, 432 (2011)
- Proapoptotic signalling through Toll-like receptor-3 involves TRIF-dependent activation of caspase-8 and is under the control of inhibitor of apoptosis proteins in melanoma cells: A. Weber, et al.; Cell Death Differ. 17, 942 (2010)
- TLR3/TICAM-1 signaling in tumor cell RIP3-dependent necroptosis: T. Seya, et al.; Oncoimmunology 1, 917 (2012)