|Solubility||Soluble in DMSO.|
|Reconstitution||Add 300µL of DMSO to obtain a 10mM stock solution. For working solution see protocol.|
|Declaration||Manufactured by ReZolve Scientific.|
|Other Product Data||
Click here for Original Manufacturer Product Data
|Shipping and Handling|
|Short Term Storage||+20°C|
|Long Term Storage||-20°C|
Protect from light.
Protect from light when in solution.
Stable for at least 1 year after receipt when stored at +20°C.
Stable for at least 2 years after receipt when stored at -20°C.
|Application Note||Download PDF|
|Product Specification Sheet|
ReZolve-ER™ is a live cell imaging agent with rapid cell uptake for imaging the endoplasmic reticulum. For use in a wide variety of live and fixed cells. This fluorophore is ideal for intermittent or long term monitoring of the endoplasmic reticulum with quick cellular uptake and localization, low cytotoxicity and high photostability to increase image acquisition time. This product is easily washed from cells allowing flexibility in experimental design. ReZolve-ER™ is ideal for fluorescence applications with excitation by UV and 405nm light sources and emission maximum of 570nm. This unique excitation/emission profile makes this product compatible with multicolour image. This product is also suitable for multiphoton imaging, further improving compatibility with live cell applications. Cell penetration and localisation of ReZolve-ER™ has been confirmed in a range of cell lines, including prostate cells (PNT2, PNT1a, LNCaP, 22RV1 and DU145), cardiomyocytes (H9c2) and neuronal cells (PC-12).
Simple and quick application
Suitable for fixed or live cell imaging
Highly resistant to photobleaching
Large stoke shift (Ex/Em 405nm / 570nm)
Compatible with other fluorescent dyes
Ideal for epi-fluorescence, confocal and multiphoton imaging
Stable at room temperature
- Imaging nuclear, endoplasmic reticulum and plasma membrane events in real time: C.A. Bader, et al.; FEBS Lett. 590, 3051 (2016)
- Bright lights down under: Metal ion complexes turning the spotlight on metabolic processes at the cellular level: T.A. Gillam, et al.; Coord. Chem. Rev. 375, 234 (2018) (Review)
- Staining the endoplasmic reticulum in combination with antibody staining: C. Bader, et al.; Nat. Protocol Exchange (2019)