Cymax TNF-α (rat) ELISA Kit

CHF 450.00
In stock
YIF-LF-EK02801 x 96 wellsCHF 450.00
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Product Details
Synonyms Il2; TCGF; IL-2; Il-2; Interleukin-2; T-Cell Growth Factor
Product Type Kit
Application Set Compound Screening
Crossreactivity Rat

1x96 wells

Sensitivity 3.159 pg/ml
Range 12.5 pg/ml - 800 pg/ml
Sample Type Buffers
Cell Culture Supernatant
Cell Lysate
Assay Type Sandwich
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

Declaration Manufactured by AbFrontier
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage +4°C
Handling Advice Any unused reconstituted standard should be discarded or frozen at -80℃.
Standard can be frozen and thawed one time only without loss of immunoreactivity.
Manual Download PDF
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Product Specification Sheet
Datasheet Download PDF

Tumor necrosis factor (TNF)-α is an important cytokine in the innate immune response produced by macrophages, neutrophils, fibroblasts, keratinocytes, NK cells, T and B cells, and tumour cells. TNF-α mediates host responses in acute and chronic inflammatory conditions, and is a mediator of protection from infection and malignancy. TNF-α causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis, and viral replication. TNF-α is initially produced as a biologically active 26 kDa transmembrane protein, which is subsequently cleaved, principally by TNF-α-converting enzyme (TACE), to release the 17kDa free protein. These proteins are arranged in biologically active homotrimers that act on the ubiquitously expressed TNF-α receptors 1 and 2 (TNFR1 and TNFR2). These 17 kDa TNF protomers are composed of two antiparallel β-pleated sheets with antiparallel β-strands, forming a 'jelly roll' β-structure, typical for the TNF family. TNF-R1 is constitutively expressed in most tissues, and can be fully activated by both the membrane-bound and soluble trimeric forms of TNF, while TNF-R2 is only found in cells of the immune system and respond to the membrane-bound form of the TNF homotrimer. Although their extracellular domains share structural and functional homology, their intracellular domains are distinct. Tumor necrosis factor promotes the inflammatory response, which in turn causes many of the clinical problems associated with autoimmune disorders such as rheumatoid arthritis, ankylosing spondylitis, Crohn's disease, psoriasis and refractory asthma. These disorders are sometimes treated by using TNF inhibitors such as infliximab (Remicade) or adalimumab (Humira). Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

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