anti-HA Tag, mAb (LF7H5)

CHF 322.00
In stock
YIF-LF-MA0048100 µlCHF 322.00
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Product Details
Synonyms HA Epitope Tag
Product Type Monoclonal Antibody
Properties
Clone LF7H5
Isotype Mouse IgG1 κ
Immunogen/Antigen Recombinant human HA-tag protein purified E. coli.
Application

ELISA
Western Blot (1:10,000~50,000)
Immunoprecipitation(1~2 μl/ml cell lysates)
ICC (1:500)

Crossreactivity All
Purity Detail Ammonium sulfate precipitation.
Formulation Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

Declaration Manufactured by AbFrontier
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
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Product Specification Sheet
Datasheet Download PDF

Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage. The expression vectors are used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. Short pieces of well defined peptides (Poly-His, Flag-epitope or cmyc epitope or HA-tag) or small proteins (bacterial GST, MBP) is often cloned along with the target gene. The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors. It has been successfully used in a variety of applications, including Western blotting, Immunoprecipitation, and immunofluorescence studies.

Product References

1) K. Terpe. (2003) Appl Microbiol Biotechnol 60: 523-533. (General)
2) Maiattis T., et. al. 1982 Molecular Cloning. (General)
3) Hopp T.P., et. al. 1988. Biotechnology 6:1204-1210. (General)
4) Smith D.B. 1988. Gene 67:31-40. (General)

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