anti-GFP, mAb (18A11)
| Product Details | |
|---|---|
| Synonyms | GFP; Green Fluorescent Protein |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | 18A11 |
| Isotype | Mouse IgG1 κ |
| Immunogen/Antigen | Recombinant green fluorescent protein. |
| Application |
ELISA |
| Crossreactivity | Human |
| Purity Detail | Ammonium sulfate precipitation. |
| Formulation | Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| Declaration | Manufactured by AbFrontier |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS | No |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Green fluorescent protein (GFP) isolated from jellyfish Aequorea aequorea is a 238 amino acid protein with an apparent molecular weight of about 27-30 kDa on SDS-PAGE. Its chromophore is formed by cyclisation and oxidation of the three amino acids Ser65, Tyr66, and Gly67. The numerous applications include, using GFP as a reporter for gene expression, as a marker to study cell lineage during development and as a tag to localize proteins in living cells. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technology has considerably contributed to a greater understanding of cellular physiology. Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin.
1) Hans-Hermann Gerdes et al. (1996) FEBS Letters 389:44-47. (General)
2) J.C. March et al. (2003) Appl Microbiol Biotechnol 62:303-315. (General)
