anti-Cytochrome C, mAb (14G6)

CHF 322.00
In stock
YIF-LF-MA0182100 µlCHF 322.00
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Product Details
Synonyms CYC; CYCS; Cytochrome c
Product Type Monoclonal Antibody
Clone 14G6
Isotype Mouse IgG1 κ
Immunogen/Antigen Recombinant human His-Cytochrome C protein purified from E. coli.
Application Western Blot (1:2,000)
Crossreactivity Human
Purity Detail Ammonium sulfate precipitation.
Formulation Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.
Other Product Data Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.
Declaration Manufactured by AbFrontier
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Product Specification Sheet
Datasheet Download PDF
Cytochrome c is a small heme protein consisting electron-transport chain in mitochondria and transfers electrons between complex III and IV. It is highly conserved through diverse species from unicellular microorganisms to animals and plants. Cytochrome c is also an intermediate in apoptosis. Currently, it is widely accepted that mitochondria play a key role in the regulation of apoptosis. In mammalian cells, a major caspase activation pathway is the cytochrome c-initiated pathway. In this pathway, a variety of apoptotic stimuli cause cytochrome c release from mitochondria. In the cytosol, cytochrome c interacts with its adaptor molecule, Apaf-1, resulting in the recruitment, processing and activation of pro-caspase-9 in the presence of dATP or ATP. Caspase-9, in turn, cleaves and activates pro-caspase-3 and -7; these effector caspases are responsible for the cleavage of various proteins leading to biochemical and morphological features characteristic of apoptosis. Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
Product References
1) Gogvadze, V. et al., (2006) Biochim Biophys Acta. 1757:639-647. (General)
2) Jiang X. and Wang X., (2004) Ann Rev Biochem. 73:87-106. (General)
3) Robertson, J.D. et al., (2000) J. Struct. Biol. 129 :346–358. (General)
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