anti-Bruton's Tyrosine Kinase, mAb (1A1)

CHF 322.00
In stock
YIF-LF-MA0191100 µlCHF 322.00
More Information
Product Details
Synonyms BPK; ATK; BTK; AGMX1; EC=2.7.10.2; Bruton Tyrosine Kinase; B-Cell Progenitor Kinase; Tyrosine-Protein Kinase BTK; Aγglobulinemia Tyrosine Kinase
Product Type Monoclonal Antibody
Properties
Clone 1A1
Isotype Mouse IgG1 κ
Immunogen/Antigen Recombinant human His/ABD-Btk protein purified from E. coli.
Application

ELISA
Western Blot (1:5,000)
IHC-P

Crossreactivity Human
Purity Detail Ammonium sulfate precipitation.
Formulation Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

Declaration Manufactured by AbFrontier
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS No
Product Specification Sheet
Datasheet Download PDF

Btk (Bruton's tyrosine kinase) is a member of the Tec family of protein tyrosine kinases (PTKs) and plays a modulatory role in many cellular processes, such as proliferation, development, differentiation, survival, and apoptosis. The Tec kinases are the second largest family of non-receptor tyrosine kinases and include Tec, Btk, Bmx, Itk, and TXK/Rlk. Mutations of Btk gene cause a primary immunodeficiency disease in humans, X-linked aγglobulinemia (XLA) which is characterized by a lack of circulating B lymphocytes and an absence of immunoglobulins as a result of defects in B cell maturation and function. Btk is found in all hematopoietic cells, with the exception of T lymphocytes and plasma cells. Btk contains amino-terminal PH (pleckstrin homology) domain which binds phosphatidylinositol (3,4,5)-trisphosphate (PIP3) helping membrane translocation upon PI3 kinase activation. The Tec kinases have similar structure of N-terminal PH domains followed by Tec homology (TH), Src homology 3 (SH3), Src homology 2 (SH2), and kinase domains. Autophosphorylation of Tyr223 in SH3 domain is necessary for full activation of Btk. Various binding proteins have been reported to interact with different domains of Btk. Plays a crucial role in B-cell ontogeny. Transiently phosphorylates GTF2I on tyrosine residues in response to B-cell receptor cross-linking. Required for the formation of functional ARID3A DNA-binding complexes.

Product References

1) Lindvall J.M. et al., (2005) Immunol Rev. 203:200-215. (General)
2) Jefferies C.A. and O’Neill L.A., (2004) Immunol Lett. 92:15-22. (General)
3) Smith C.I. et al., (2001) Bioessays, 23:436-446. (General)
4) Schaeffer E.M. and Schwartzberg P.L., (2000) Curr Opin Immunol. 12:282-288. (General)

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