anti-PLK1, mAb (18D4)

CHF 322.00
In stock
YIF-LF-MA0194100 µlCHF 322.00
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Product Details
Synonyms PLK; PLK1; PLK-1; STPK13; EC=2.7.11.21; Polo-like Kinase 1; Serine/Threonine-Protein Kinase 13; Serine/Threonine-Protein Kinase PLK1
Product Type Monoclonal Antibody
Properties
Clone 18D4
Isotype Mouse IgG1 κ
Immunogen/Antigen Recombinant human GST/His-Plk1 protein purified from E. coli.
Application

ELISA
Western Blot (1:1,000)
Immunoprecipitation (1 μl)

Crossreactivity Human
Mouse
Rat
Purity Detail Ammonium sulfate precipitation.
Formulation Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

Declaration Manufactured by AbFrontier
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS No
Product Specification Sheet
Datasheet Download PDF

Polo-like kinases (Plks) are important regulators of the cell cycle. Plk1, the most studied Plk, has been implicated in regulating centrosome maturation, mitotic entry, sister chromatid cohesion, the anaphase-promoting complex/cyclosome (APC/C), and cytokinesis. Sister chromatid separation and the subsequent formation of two genetically identical daughter cells depend on the symmetrical attachment of all chromosomes to the mitotic spindle, a process called chromosome biorientation. Several mitosis-specific protein kinases have been implicated in bipolar spindle assembly and chromosome biorientation. For example, Cdk1 (cyclin-dependent kinase 1) is known to phosphorylate Eg5, a kinesin that is required for the migration of centrosomes and is thus needed for the formation of bipolar spindles. At the onset of mitosis, Plks contribute to the activation of Cdk1-cyclinB, and they are also required for the inactivation of Cdk1 and exit from mitosis. Plks are important regulators of the APC/C, a key component of the ubiquitin-dependent proteolytic degradation pathway. Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis.

Product References

1) Sumara I. et al., (2004) Curr Biol. 14:1712-1722. (General)
2) Nigg E.A., (1999) Curr Opin Cell Biol. 10:776-783. (General)

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