anti-ZAP70, mAb (49B4)

CHF 322.00
In stock
YIF-LF-MA0197100 µlCHF 322.00
More Information
Product Details
Synonyms SRK; ZAP70; EC=; Syk-related Tyrosine Kinase; Tyrosine-Protein Kinase ZAP-70; 70 kDa zeta-Chain Associated Protein
Product Type Monoclonal Antibody
Clone 49B4
Isotype Mouse IgG1 κ
Immunogen/Antigen Recombinant human ABD-ZAP70 protein purified from E. coli.
Application ELISA
Western Blot (1:10,000)
Immunoprecipitation (2 μl)
Crossreactivity Human
Purity Detail Ammonium sulfate precipitation.
Formulation Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.
Other Product Data Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.
Declaration Manufactured by AbFrontier
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Product Specification Sheet
Datasheet Download PDF
ζ-chain associated protein kinase, ZAP70, is a 70 kDa member of the Syk family kinase predominantly involved in T cell receptor (TCR) signaling. It is structurally homologous to Syk, a PTK that is involved in proximal BCR signaling. ZAP-70 is a key signaling molecule in T cell activation and also plays a role in apoptosis and cell migration. SYK family tyrosine kinases contain a C-terminal kinase domain and tandem N-terminal SH2 domains that bind phosphorylated ITAMs (immunoreceptor tyrosine-based activation motif). Linker region that contains multiple tyrosines separates the SH2 domains from the kinase domain. Phosphorylated tyrosines act as docking sites for phospholipase Cγ1 (PLCγ1). ZAP-70 and Syk are functionally homologous in antigen receptor signaling. Expression of ZAP-70 in Syk− B cells reconstitutes SCR function. Reconstitution requires the presence of functional Src homology 2 (SH2) and catalytic domains of ZAP-70. Expression of ZAP-70 is an important negative prognostic factor in chronic lymphocytic leukemia (CLL) with more rapid disease progression and shorter survival. Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
Product References
1) Gobessi S. et al., (2007) Blood. 109:2032-2039. (General)
2) Orchard J.A. et al., (2004) Lancet. 363:105-111. (General)
3) Kong G.H. et al., (1995) Immunity. 2:485-492. (General)
© 2017 Adipogen Life Sciences. Pictures: © 2012 Martin Oeggerli. All Rights Reserved.