anti-ZAP70, mAb (49B4)

CHF 315.00
In stock
YIF-LF-MA0197100 µlCHF 315.00
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Product Details
Synonyms SRK; ZAP70; EC=; Syk-related Tyrosine Kinase; Tyrosine-Protein Kinase ZAP-70; 70 kDa zeta-Chain Associated Protein
Product Type Monoclonal Antibody
Clone 49B4
Isotype Mouse IgG1 κ
Immunogen/Antigen Recombinant human ABD-ZAP70 protein purified from E. coli.

Western Blot (1:10,000)
Immunoprecipitation (2 μl)

Crossreactivity Human
Purity Detail Ammonium sulfate precipitation.
Formulation Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.
Isotype Negative Control

Mouse IgG1 Isotype Control

Other Product Data

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Our product description may differ slightly from the original manufacturers product datasheet.

Declaration Manufactured by AbFrontier
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
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Product Specification Sheet
Datasheet Download PDF

ζ-chain associated protein kinase, ZAP70, is a 70 kDa member of the Syk family kinase predominantly involved in T cell receptor (TCR) signaling. It is structurally homologous to Syk, a PTK that is involved in proximal BCR signaling. ZAP-70 is a key signaling molecule in T cell activation and also plays a role in apoptosis and cell migration. SYK family tyrosine kinases contain a C-terminal kinase domain and tandem N-terminal SH2 domains that bind phosphorylated ITAMs (immunoreceptor tyrosine-based activation motif). Linker region that contains multiple tyrosines separates the SH2 domains from the kinase domain. Phosphorylated tyrosines act as docking sites for phospholipase Cγ1 (PLCγ1). ZAP-70 and Syk are functionally homologous in antigen receptor signaling. Expression of ZAP-70 in Syk− B cells reconstitutes SCR function. Reconstitution requires the presence of functional Src homology 2 (SH2) and catalytic domains of ZAP-70. Expression of ZAP-70 is an important negative prognostic factor in chronic lymphocytic leukemia (CLL) with more rapid disease progression and shorter survival. Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.

Product References

1) Gobessi S. et al., (2007) Blood. 109:2032-2039. (General)
2) Orchard J.A. et al., (2004) Lancet. 363:105-111. (General)
3) Kong G.H. et al., (1995) Immunity. 2:485-492. (General)

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