anti-IkBα, mAb (7A4)

CHF 322.00
In stock
YIF-LF-MA0235100 µlCHF 322.00
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Product Details
Synonyms IKBA; NFKBIA; IkB-α; IκBα; I-κ-B-α; NF-κ-B Inhibitor α; Major Histocompatibility Complex Enhancer-binding Protein MAD3
Product Type Monoclonal Antibody
Properties
Clone 7A4
Isotype Mouse IgG1 κ
Immunogen/Antigen Recombinant human His/ABD-IkBα protein purified from E. coli.
Application

ELISA
Western Blot (1:2,000)

Crossreactivity Human
Mouse
Rat
Purity Detail Ammonium sulfate precipitation.
Formulation Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

Declaration Manufactured by AbFrontier
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS No
Product Specification Sheet
Datasheet Download PDF

IκBα is the major IκB protein member of IκB family that function to inhibit the NF-κB transcription factor. IκBs have an N-terminal regulatory domain, followed by six or more ankyrin repeats and a PEST domain near their C terminus. IκBα inhibits NF-κB by masking the nuclear localization signals (NLS) of NF-κB proteins and keeping them in an inactive state in the cytoplasm. In addition, IκBα blocks the ability of NF-κB transcription factors to bind to DNA, which is required for NF-κB's proper functioning. Upon stimulation by the proinflammatory cytokine tumor necrosis factor (TNF), signaling pathways lead to activation of the subunit of the IκB kinase (IKK) complex, which then phosphorylates IκB proteins on two N-terminal serine residues. The IKK is an unusual kinase in that it contains two related kinases, IKKα and IKKβ, and a regulatory subunit, NEMO (NF-κB essential modifier). The primary role of NF-κB is to maintain normal cellular functions that range from cell-to-cell communication to cell motility, cell cycle progression, and cell lineage development. Inhibits the activity of dimeric NF-κ-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.

Product References

1) Gilmore TD, (2006) Oncogene. 25(51) :6680–6684. (General)
2) Hayden MS and Ghosh S, (2004) Genes Dev. 18(18):2195-2224. (General)
3) Gilmore TD, (1999) Oncogene. 18(49):6842-6844. (General)

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