anti-Myoglobin, mAb (15H1)

CHF 322.00
In stock
YIF-LF-MA0243100 µlCHF 322.00
More Information
Product Details
Synonyms MB; Myoglobin
Product Type Monoclonal Antibody
Properties
Clone 15H1
Isotype Mouse IgG1 κ
Immunogen/Antigen Recombinant human His-Myoglobin protein purified from E. coli.
Application

ELISA
Western Blot (1:2,000)

Crossreactivity Human
Purity Detail Ammonium sulfate precipitation.
Formulation Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

Declaration Manufactured by AbFrontier
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF

Myoglobin is a cytoplasmic hemoprotein, expressed solely in cardiac myocytes and oxidative skeletal muscle fibers, that reversibly binds O2 by its heme (iron-containing porphyrin) prosthetic group in the center around which the remaining apoprotein folds. The red color that meat takes is partly determined by the charge of the iron atom in myoglobin and the oxygen attached to it. When meat is in its raw state, the iron atom has a charge of +2 and is bound to an oxygen molecule. Myoglobin is a single-chain globular protein of 153 amino acids and has a molecular weight of 16,700 daltons. Unlike the blood-borne hemoglobin, to which it is structurally related, this protein does not exhibit cooperative binding of oxygen, since positive cooperaivity is a property of multimeric/oligomeric proteins only. Recent gene disruption studies using the myoglobin deficient mouse model support the conclusion that the functions of myoglobin include: facilitated oxygen transport, the storage of oxygen and a scavenger of nitric oxide or reactive oxygen species. Myoglobin is an early indicator of cardiac ischemia that achieves maximal sensitivity 3 to 4 hours after symptom onset. However, it is only marginally more sensitive than CK-MB early after the onset of symptoms, and it is less sensitive 6 to 8 hours after symptom onset. Because of rapid clearance from the blood, myoglobin may "miss" late-presenting patients, and it is less cardiospecific than CK-MB. Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.

Product References

1) Garry DJ and Mammen PP. (2007) Adv Exp Med Biol. 618:181-193. (General)
2) Lippi G et al. (2006) CJEM. 8(1):27-31 (General)
3) Ordway GA and Garry DJ. (2004) J Exp Biol. 207(Pt 20):3441-3446. (General)

© 2017 Adipogen Life Sciences. Pictures: © 2012 Martin Oeggerli. All Rights Reserved.