anti-NF-κB p52, mAb (9D2)

CHF 322.00
In stock
YIF-LF-MA0261100 µlCHF 322.00
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Product Details
Synonyms NFKB2; LYT10
Product Type Monoclonal Antibody
Properties
Clone 9D2
Isotype Mouse IgG2b κ
Immunogen/Antigen Recombinant human His-NFκB (aa 1-454) protein purified from E. coli.
Application

ELISA
Western Blot (1:2,000)

Crossreactivity Human
Mouse
Rat
Purity Detail Ammonium sulfate precipitation.
Formulation Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

Declaration Manufactured by AbFrontier
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
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Product Specification Sheet
Datasheet Download PDF

NF-κB (Nuclear Factor κ B) is a nuclear transcription factor found in all cell types and is involved in cellular responses to stimuli such as stress, cytokines, free radicals, ultraviolet irradiation, and bacterial or viral antigens. NF-κB plays a key role in regulating the immune response to infection. Consistent with this role, incorrect regulation of NF-κB has been linked to cancer, inflammatory and autoimmune diseases, septic shock, viral infection and improper immune development. There are five members in the NF-κB family: NF-κB1, NF-κB2, RelA (also named p65), RelB, and c-Rel. The most common form of NF-κB is the p50/RelA heterodimer, although other forms of NF-κB dimers, such as p50/p50, p52/p52, p52/RelA, p50/c-Rel, c-Rel/c-Rel, p52/RelB, and p50/RelB, have also been identified in some types of cells. The primary role of NF-κB is to maintain normal cellular functions that range from cell-to-cell communication to cell motility, cell cycle progression, and cell lineage development. The activity of NF-κB is tightly regulated by interaction with inhibitory IκB proteins. NF-κ-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-κ-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at κ-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different κ-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-κ-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-κ-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-κ-B inhibitor (I-κ-B) family. In a conventional activation pathway, I-κ-B is phosphorylated by I-κ-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-κ-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-κ-B RelB-p52 complexes. The NF-κ-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-κ-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-κ-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the κ-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-κ-B protein complex, which stimulates the HIV enhancer in synergy with p65.

Product References

1) Campbell KJ, Perkins ND. Cell Cycle. (2004) vol.3(7): p.869-72. (General)
2) Perkins ND. Trends Cell Biol. (2004) vol.14(2): p.64-9. (General)
3) Zanetti M, et al, Ann N Y Acad Sci. (2003) vol.987: p.249-57. (General)

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