anti-Vimentin, mAb (35E2)

CHF 315.00
In stock
YIF-LF-MA0265100 µlCHF 315.00
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Product Details
Synonyms VIM; Vimentin
Product Type Monoclonal Antibody
Clone 35E2
Isotype Mouse IgG1 κ
Immunogen/Antigen Recombinant human His-Vimentin protein purified from E. coli.

Western Blot (1:2,000)

Crossreactivity Human
Purity Detail Ammonium sulfate precipitation.
Formulation Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.
Isotype Negative Control

Mouse IgG1 Isotype Control

Other Product Data

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Our product description may differ slightly from the original manufacturers product datasheet.

Declaration Manufactured by AbFrontier
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
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Product Specification Sheet
Datasheet Download PDF

Vimentin is a member of the intermediate filament family of proteins found in various non-epithelial cells, especially mesenchymal cells. Vimentin is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. Vimentin plays a significant role in supporting and anchoring the position of the organelles in the cytosol. Although most intermediate filaments are stable structures, vimentin also has a dynamic nature which is important when offering flexibility to the cell. Two monomers which have central α-helical domains, capped on each end by non-helical domains twist around each other to form a coiled-coil dimer. Two dimers then form a tetramer, which, in turn, form a sheet by interacting with other tetramers. There are some reports related to the biochemical function of intermediate filament network. The intracellular movement of LDL-derived cholesterol from the lysosome to the site of esterification is a vimentin-dependent process. A role for vimentin in mechanotransduction of shear stress has also been suggested. The mechanical stress of fluid shear on endothelial cells seems to trigger MAPK signaling pathways and stimulates proliferation. Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.

Product References

1) Pekny M and Lane EB, (2007) Exp Cell Res. 313(10):2244-2254 (General)
2) Holwell TA et al., (1999) J Lipid Res. 40(8):1440-1452. (General)
3) Henrion D et al., (1997) J. Clin. Invest. 100:2909–2914 (General)
4) Sarria AJ et al., (1992) J Biol Chem. 267(27):19455-19463. (General)

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