anti-UCHL3, mAb (AF9G8)

CHF 315.00
In stock
YIF-LF-MA0312100 µlCHF 315.00
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Product Details
Synonyms UCHL3; UCH-L3; EC=; Ubiquitin Thioesterase L3; Ubiquitin Carboxyl-terminal Hydrolase Isozyme L3
Product Type Monoclonal Antibody
Clone AF9G8
Isotype Mouse IgG2b κ
Immunogen/Antigen Recombinant human GST-UCHL3 protein purified from E. coli.

Western Blot (1:2,000)

Crossreactivity Human
Purity Detail Ammonium sulfate precipitation.
Formulation Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.
Isotype Negative Control

Mouse IgG2b Isotype Control

Other Product Data

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Our product description may differ slightly from the original manufacturers product datasheet.

Declaration Manufactured by AbFrontier
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
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Product Specification Sheet
Datasheet Download PDF

The two ubiquitin C-terminal hydrolase (UCH) enzymes, UCHL1 and UCHL3, deubiquitinate ubiquitin-protein conjugates and control the cellular balance of ubiquitin. UCHL1 and UCHL3 are both small proteins of ~220 amino acids that share more than 40% amino acid sequence identity. UCHL3 is universally expressed in all tissues, while UCHL1 is expressed exclusively in neuronal tissue, testis and ovary. The activity of UCHL3 is more than 200 fold higher than UCHL1 when a fluorogenic ubiquitin substrate is used. UCHL1 associates with monoubiquitin and UCHL3 binds to Nedd8, ubiquitin-like protein. UCHL1 and UCHL3 play a role in the regulation of neuronal development and spermatogenesis. UCHL1 is involved in the pathogenesis of Parkinson’s disease (PD) and Alzheimer’s disease (AD). Down-regulation and extensive oxidative modification of UCHL1 have been observed in the brains of AD patients as well as PD patients. Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8.

Product References

1) Osaka H et al., (2003) Human Molecular Genetics 12(16):1945–1958. (General)
2) Kwon J, (2007) Exp Anim 56:71-7. (General)
3) Sakurai M et al., (2006) J Cell Sci 119:162-171. (General)

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