anti-Casein Kinase 1 ε, mAb (AF6C1)

CHF 322.00
In stock
YIF-LF-MA0313100 µlCHF 322.00
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Product Details
Synonyms CKIe; CSNK1E; EC=2.7.11.1; CKI-ε; Casein Kinase I Isoform ε
Product Type Monoclonal Antibody
Properties
Clone AF6C1
Isotype Mouse IgG1 κ
Immunogen/Antigen Recombinant human His-Casein Kinase Ie protein purified from E. coli.
Application

ELISA
Western Blot (1:2,000)
Immunoprecipitation (1 μl/400 μl lysate)

Crossreactivity Human
Mouse
Rat
Purity Detail Ammonium sulfate precipitation.
Formulation Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

Declaration Manufactured by AbFrontier
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS No
Product Specification Sheet
Datasheet Download PDF

Casein kinases are a group of ubiquitous Ser/Thr kinases that phosphorylate key regulatory proteins involved in the control of cell differentiation, proliferation, chromosome segregation, circadian rhythms, and metabolic pathways. CK1 family members share a highly conserved kinase domain but differ in their variable N- and C-terminal domains. Mammals have seven family members: α, β, γ1, γ2, γ3, δ and ε. They all share at least 50% amino acid identity within the protein kinase catalytic domain. CK1 isoforms exclusively use ATP as phosphate donor and are generally co-factor independent. Casein kinase I ε is a Wnt-regulated kinase, and regulated phosphorylation of Dvl allows fine tuning of the Wnt-b-catenin signaling pathway. CKIe positively regulates Akt possibly by inhibiting PP2A. CKIe play an important role in neurodegenerative diseases. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates DVL1. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm. Inhibits cytokine-induced granuloytic differentiation

Product References

1) Klimowski LK et al., (2006) FEBS J 273(20):4594-602. (General)
2) Knippschild U et al., (2005) Cell Signal 17(6):675-89. (General)
3) Hino S et al, (2003) J Biol Chem 278(16):14066-73. (General)

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