anti-LDHB, mAb (AF21F4)

Specifications / Handling

More Information
Product Details
Synonyms	LDHB; LDH-B; LDH-H; EC=1.1.1.27; LDH Heart Subunit; L-Lactate Dehydrogenase B Chain; Renal Carcinoma Antigen NY-REN-46
Product Type	Monoclonal Antibody
Properties
Clone	AF21F4
Isotype	Mouse IgG2b κ
Immunogen/Antigen	Recombinant human His-LDHB protein purified from E. coli.
Application	ELISA Western Blot (1:2,000~5,000)
Crossreactivity	Human Mouse Rat
Purity Detail	Ammonium sulfate precipitation.
Formulation	Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.
Other Product Data	Click here for Original Manufacturer Product Datasheet Our product description may differ slightly from the original manufacturers product datasheet.
Declaration	Manufactured by AbFrontier
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS	Inquire
Product Specification Sheet
Datasheet	Download PDF

Description

Lactate dehydrogenase (LDH) is the enzyme in the glycolytic pathway that converts pyruvate to lactate with concomitant interconversion of NADH and NAD+. In mammals, the enzyme is encoded by three genes: LDHA (M or muscle form), LDHB (H or heart form), and LDHC (X or testis form). The three human LDHs have 84–89% sequence similarities and 69–75% amino acid identities. There are five different LDH isoenzymes based on the proportion of M and H chains existing in the LDH tetrameric structure. The LDHA is hypoxia inducible and its expression is directly controlled by the transcriptional activity of the hypoxia inducible factor 1α (HIF1α). LDHB is also known to be upregulated in many cancers.

Product References

1) Mdluli K et al., (2005) Biochim Biophys Acta 1753(2):209-16. (General)
2) Xie H et al, (2009) Mol Cancer Ther 8(3):626-35. (General)
3) Giatromanolaki A et al, (2008) Leuk Lymphoma 49(11) :2181-6. (General)

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