anti-Calretinin (Calbindin-2), mAb (AF5D11)

CHF 322.00
In stock
YIF-LF-MA0346100 µlCHF 322.00
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Product Details
Synonyms CR; CALB2; CAB29; Calretinin; 29 kDa Calbindin
Product Type Monoclonal Antibody
Properties
Clone AF5D11
Isotype Mouse IgG1 κ
Immunogen/Antigen Recombinant human His-Calbindin2 protein purified from E. coli.
Application

Western Blot (1:1,000)

Crossreactivity Human
Mouse
Purity Detail Ammonium sulfate precipitation.
Formulation Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

Declaration Manufactured by AbFrontier
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS No
Product Specification Sheet
Datasheet Download PDF

Calcium binding proteins (CBPs) regulate intracellular levels of calcium Ca2+ ions. CBPs are involved in numerous functions, such as cell signaling, calcium uptake and transport, and cell motility. Calretinin (CR), calbindin D-28k (CB) and parvalbumin (PV) belong to the large family of Efhand calcium-binding proteins. The EF-hand is an amino acid sequence with a haracteristic threedimensional helix-loop-helix structure with high affinity for Ca2+. Calbindin D28k (calbindin) is a hexa EF-hand protein of 261 residues with 59% sequence identity to calretinin. It is a single-domain protein and the six EF hands interact extensively with one another so that they form one large globular domain. Calbindin is found in the brain and central nervous system with a distinct distribution over certain neuronal subtypes. Neuroprotective effects of calbindin has been reported in ischemic and glutamate toxicity models, primarily due to its ability to chelate calcium. Mice lacking calbindin have been shown to suffer from impaired motor coordination due to altered neuronal Ca2+-homeostasis. Notably, calbindin is found in high concentration in the kidney and in pancreatic β-cells, where it has been shown to protect against apoptosis. In osteoblasts, anti-apoptic activity of calbindin through the binding to caspase-3 has also been reported. Calbindin protects against apoptotic and necrotic cell death, suggesting its ability to buffer calcium. Calbindin has been reported to binds Zn2+, revealing negative allosteric effect between the Zn2+- and Ca2+-binding events. Calretinin is a calcium-binding protein which is abundant in auditory neurons.

Product References

1) Bauer MC et al., (2008) Protein Sci 17(4):760-767. (General)
2) Anelli R and Heckman CJ, (2005) J Neurocytol 34(6):369-385. (General)
3) Linse S et al., (1997) Protein Sci 6(11) :2385-2396. (General)

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