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anti-Calretinin (Calbindin-2), mAb (AF5D11)
Product Details | |
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Synonyms | CR; CALB2; CAB29; Calretinin; 29 kDa Calbindin |
Product Type | Monoclonal Antibody |
Properties | |
Clone | AF5D11 |
Isotype | Mouse IgG1 κ |
Immunogen/Antigen | Recombinant human His-Calbindin2 protein purified from E. coli. |
Application |
Western Blot (1:1,000) |
Crossreactivity |
Human Mouse |
Purity Detail | Ammonium sulfate precipitation. |
Formulation | Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. |
Isotype Negative Control | |
Other Product Data |
Click here for Original Manufacturer Product Datasheet |
Declaration | Manufactured by AbFrontier |
Shipping and Handling | |
Shipping | BLUE ICE |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
Documents | |
MSDS | Inquire |
Product Specification Sheet | |
Datasheet |
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Calcium binding proteins (CBPs) regulate intracellular levels of calcium Ca2+ ions. CBPs are involved in numerous functions, such as cell signaling, calcium uptake and transport, and cell motility. Calretinin (CR), calbindin D-28k (CB) and parvalbumin (PV) belong to the large family of Efhand calcium-binding proteins. The EF-hand is an amino acid sequence with a haracteristic threedimensional helix-loop-helix structure with high affinity for Ca2+. Calbindin D28k (calbindin) is a hexa EF-hand protein of 261 residues with 59% sequence identity to calretinin. It is a single-domain protein and the six EF hands interact extensively with one another so that they form one large globular domain. Calbindin is found in the brain and central nervous system with a distinct distribution over certain neuronal subtypes. Neuroprotective effects of calbindin has been reported in ischemic and glutamate toxicity models, primarily due to its ability to chelate calcium. Mice lacking calbindin have been shown to suffer from impaired motor coordination due to altered neuronal Ca2+-homeostasis. Notably, calbindin is found in high concentration in the kidney and in pancreatic β-cells, where it has been shown to protect against apoptosis. In osteoblasts, anti-apoptic activity of calbindin through the binding to caspase-3 has also been reported. Calbindin protects against apoptotic and necrotic cell death, suggesting its ability to buffer calcium. Calbindin has been reported to binds Zn2+, revealing negative allosteric effect between the Zn2+- and Ca2+-binding events. Calretinin is a calcium-binding protein which is abundant in auditory neurons.
1) Bauer MC et al., (2008) Protein Sci 17(4):760-767. (General)
2) Anelli R and Heckman CJ, (2005) J Neurocytol 34(6):369-385. (General)
3) Linse S et al., (1997) Protein Sci 6(11) :2385-2396. (General)