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anti-CDC25A, mAb (PK1-AF2D12)
Product Details | |
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Synonyms | CDC25A; EC=3.1.3.48; M-Phase Inducer Phosphatase 1; Dual Specificity Phosphatase Cdc25A |
Product Type | Monoclonal Antibody |
Properties | |
Clone | PK1-AF2D12 |
Isotype | Mouse IgG1 κ |
Immunogen/Antigen | Recombinant human protein purified from E. coli. |
Application |
Western Blot (1:2,000) |
Crossreactivity | Human |
Purity Detail | Ammonium sulfate precipitation. |
Formulation | Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. |
Isotype Negative Control | |
Other Product Data |
Click here for Original Manufacturer Product Datasheet |
Declaration | Manufactured by AbFrontier |
Shipping and Handling | |
Shipping | BLUE ICE |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
Documents | |
MSDS | Inquire |
Product Specification Sheet | |
Datasheet |
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The Cdc25 phosphatases are key regulators of normal cell division and the cell's response to DNA damage. Cdc25 isoforms are dual-specificity phosphatases that remove inhibitory phosphates from Thr14 and Tyr15 in Cdk1 and Cdk2. Cdk1 is necessary for mitotic onset and shares an overlapping role with Cdk2 in controlling S phase initiation. Cdc25 isoforms are also key targets of the Chk1 and Chk2 checkpoint kinases, which inactivate Cdc25 to halt cell cycle progression when DNA is damaged or incompletely replicated. Mammalian cells have three Cdc25 isoforms: Cdc25A, Cdc25B and Cdc25C. Cdc25A, which regulates both early and late cell-cycle transitions, controls progression through S phase and entry into mitosis, whereas Cdc25B and Cdc25C primarily control entry into mitosis. Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
1) Karlsson-Rosenthal C and Millar JB, (2006) Trends Cell Biol 16(6):285-92. (General)
2) Boutros R, et al., (2006) Curr Opin Cell Biol 18(2):185-91. (General)
3) Donzelli M and Draetta GF, (2003) EMBO 4(7):671-7. (General)