anti-CDC25A, mAb (PK1-AF2D12)

CHF 322.00
In stock
YIF-LF-MA0347100 µlCHF 322.00
More Information
Product Details
Synonyms CDC25A; EC=3.1.3.48; M-Phase Inducer Phosphatase 1; Dual Specificity Phosphatase Cdc25A
Product Type Monoclonal Antibody
Properties
Clone PK1-AF2D12
Isotype Mouse IgG1 κ
Immunogen/Antigen Recombinant human protein purified from E. coli.
Application

Western Blot (1:2,000)

Crossreactivity Human
Purity Detail Ammonium sulfate precipitation.
Formulation Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

Declaration Manufactured by AbFrontier
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF

The Cdc25 phosphatases are key regulators of normal cell division and the cell's response to DNA damage. Cdc25 isoforms are dual-specificity phosphatases that remove inhibitory phosphates from Thr14 and Tyr15 in Cdk1 and Cdk2. Cdk1 is necessary for mitotic onset and shares an overlapping role with Cdk2 in controlling S phase initiation. Cdc25 isoforms are also key targets of the Chk1 and Chk2 checkpoint kinases, which inactivate Cdc25 to halt cell cycle progression when DNA is damaged or incompletely replicated. Mammalian cells have three Cdc25 isoforms: Cdc25A, Cdc25B and Cdc25C. Cdc25A, which regulates both early and late cell-cycle transitions, controls progression through S phase and entry into mitosis, whereas Cdc25B and Cdc25C primarily control entry into mitosis. Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

Product References

1) Karlsson-Rosenthal C and Millar JB, (2006) Trends Cell Biol 16(6):285-92. (General)
2) Boutros R, et al., (2006) Curr Opin Cell Biol 18(2):185-91. (General)
3) Donzelli M and Draetta GF, (2003) EMBO 4(7):671-7. (General)

© 2017 Adipogen Life Sciences. Pictures: © 2012 Martin Oeggerli. All Rights Reserved.