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Tumor Necrosis Factor α (human) (rec.)
Product Details | |
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Synonyms | Tumor Necrosis Factor Ligand Superfamily Member 2; Cachetin |
Product Type | Protein |
Properties | |
Source/Host | E. coli |
Sequence | Human TNF-alpha (aa77-233). |
Crossreactivity | Human |
Biological Activity | The ED50 as determined by the cytolysis of murine L929cells in the presense of actinomycin D is 0.03-0.06ng/ml |
MW | 17.5kDa |
Purity | ≥98% (SDS-PAGE) |
Endotoxin Content | <0.05EU/µg purified protein (LAL test; Lonza). |
Accession Number | P01375 |
Formulation | Liquid. In 20mM Tris, pH 8.0, 1mM EDTA, 10% Glycerol. |
Other Product Data |
Click here for Original Manufacturer Product Datasheet |
Declaration | Manufactured by AbFrontier. |
Shipping and Handling | |
Shipping | BLUE ICE |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice | Avoid freeze/thaw cycles. |
Use/Stability | After reconstitution, store at -80°C. |
Documents | |
MSDS | Inquire |
Product Specification Sheet | |
Datasheet |
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Tumor necrosis factor-a (TNF-a) is a pleiotropic pro-inflammatory cytokine produced mainly by activated macrophages and in smaller amounts by several other types of cell. TNF-a has been isolated and characterized from a large number of mammals and fish species. TNF-a is expressed in two different forms: a soluble. Mature 17kDa form and a transmembrane 26kDa form. TNF-a interacts with two distinct cell-surface receptors : Tumor necrosis factor receptor 1(TNFR1) and TNFR2. these mediate the cellular actions. The actions of TNF-a are diverse and profound involving inflammation, apoptosis, cell proliferation and the stimulation of various aspects within the immune system. TNF-a is increasingly recognized as a key regulator of lipid metabolism in adipose tissue and protein catabolism in muscle and in disease states such as cancer, Acquired Immune Deficiency Syndrome (AIDS) and obesity-related insulin resistance.
1) Wen-Xing Ding et al (2004) J.Cell. Mol. Med 8(4);445-454. 2) Sudhir Gupta (2002) Journal of Clinical Immunology 22(4);185-194. 3) Frederick W. goetz et al (2004) Developmental and comparative Immunology 28;487-497. 4) Havell, E.A. (1987) J. Immunol. 39:4225-4231.