Tumor Necrosis Factor α (human) (rec.)
| Product Details | |
|---|---|
| Synonyms | TNF; TNFA; TNF-α |
| Product Type | Protein |
| Properties | |
| Source/Host | E. coli |
| Crossreactivity | Human |
| Biological Activity |
The ED50 as determined by the cytolysis of murine L929 cells in the presense of actinomycin D is 0.03-0.06 ng/ml. |
| Purity | ≥98% (SDS-PAGE) |
| Formulation | Liquid in 20mM Tris, pH 8.0, 1mM EDTA, 10% Glycerol. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| Declaration | Manufactured by AbFrontier |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -20°C |
| Long Term Storage | -80°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability | After reconstitution, store at -80°C. |
| Documents | |
| MSDS | No |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Tumor necrosis factor-α (TNF-α) is a pleiotropic pro-inflammatory cytokine produced mainly by activated macrophages and in smaller amounts by several other types of cell. TNF-α has been isolated and characterized from a large number of mammals and fish species. TNF-α is expressed in two different forms: a soluble. Mature 17kDa form and a transmembrane 26kDa form. TNF-α interacts with two distinct cell-surface receptors, Tumor necrosis factor receptor 1 (TNFR1) and TNFR2. these mediate the cellular actions. The actions of TNF-α are diverse and profound involving inflammation, apoptosis, cell proliferation and the stimulation of various aspects within the immune system. TNF-α is increasingly recognized as a key regulator of lipid metabolism in adipose tissue and protein catabolism in muscle and in disease states such as cancer, Acquired Immune Deficiency Syndrome (AIDS) and obesity-related insulin resistance.
1) Wen-Xing Ding et al (2004) J.Cell. Mol. Med 8(4);445-454. (General)
2) Sudhir Gupta (2002) Journal of Clinical Immunology 22(4);185-194. (General)
3) Frederick W. goetz et al (2004) Developmental and comparative Immunology 28;487-497. (General)
4) Havell, E.A. (1987) J. Immunol. 39:4225-4231. (General)
