CKBB (human) (rec.)

CHF 366.00
In stock
YIF-LF-P00910.2 mgCHF 366.00
 
More Information
Product Details
Synonyms CKB; B-CK; CKBB; EC=2.7.3.2; Creatine Kinase B-type; Creatine Kinase B Chain
Product Type Protein
Properties
Source/Host E. coli
Crossreactivity Human
Purity ≥95% (SDS-PAGE)
Formulation Lyophilized from 20mM HEPES, pH7.0.
Other Product Data Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.
Declaration Manufactured by AbFrontier
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Use/Stability After reconstitution, store at -80°C.
Documents
MSDS No
Product Specification Sheet
Datasheet Download PDF
Creatine kinase (CK), also known as phosphocreatine kinase or creatine phosphokinase (CPK) is an enzyme expressed by various tissue types. It catalyzes the reversible transfer of the N-phosphoryl group from phosphocreatine (PCr) to ADP to regenerate ATP. Creatine kinase plays a key role in the energy homeostasis of cells with intermittently high, fluctuating energy requirements, such as skeletal and cardiac muscle cells, neurons, photoreceptors, spermatozoa and electrocytes. Creatine kinase consists of two subunits, which can be either B (brain type) or M (muscle type). Therefore, three different cytosolic isoenzymes exist: CK-MM, CK-BB and CK-MB. Cytosolic CK isoenzymes are always co-expressed in a tissue-specific fashion together with a mitochondrial isoform. Skeletal muscle expresses CK-MM (98%) and low levels of CK-MB (1%). The heart muscle expresses CK-MM at 70% and CK-MB at 25-30%. CK-BB is expressed in all tissues at low levels. Cytosolic CKs, in close conjunction with Ca2+-pumps, play a crucial role for the energetics of Ca2+-homeostasis. Octameric mitochondrial Mi-CK binds and crosslinks mitochondrial membranes. The CK system is regulated by AMP-activated protein kinase via PCr/Cr and ATP/AMP ratios. The cardiac-specific isoenzyme of creatine kinase, CK-MB, is a biomarker for myocardial infarction along with other markers such as cardiac Troponin I and myoglobin. The introduction of immunologic mass determination of CK-MB was a major breakthrough that replaced the traditional enzymatic assay.
Product References
1) Brown AM et al., (2007) Ann Emerg Med. 49(2):153-163. (General)
2) Lippi G et al., (2006) CJEM. 8(1):27-31. (General)
3) Newby L, (2004) Prog Cardiovasc Dis. 46(5):404-416. (General)
4) Wallimann T et al., (1998) Biofactors. 8(3-4):229-234. (General)
© 2017 Adipogen Life Sciences. Pictures: © 2012 Martin Oeggerli. All Rights Reserved.