G-CSF (human) (rec.)
|Synonyms||CSF3; G-CSF; C17orf33; Filgrastim; Lenograstim; Pluripoietin; Granulocyte Colony-stimulating Factor|
The ED50 of G-CSF is typically 5-40 pg/ml. Measured in a cell proliferation assay using a murine myeloblastic cell line, NFS-60.
|Formulation||Liquid in PBS, 10% glycerol.|
|Other Product Data||
Click here for Original Manufacturer Product Datasheet
|Declaration||Manufactured by AbFrontier|
|Shipping and Handling|
|Short Term Storage||-20°C|
|Long Term Storage||-80°C|
|Handling Advice||Avoid freeze/thaw cycles.|
|Product Specification Sheet|
Granulocyte colony-stimulating factor (G-CSF) is a hematopoietic cytokine that promotes the proliferation and differentiation of cells in the granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-α and IFN-γ. Other cells can also secrete G-CSF after IL-1β,TNF-α and LPS activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. G-CSF has a effect in the treatment of leukopenia, AIDS, MDS and bone marrow transplantation. In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid precursor proteins, have been isolated. Both protein have identical amino acid sequences except for a three amino acid insertion (deletion) at the 35th amino acid residue from the N-terminus of the mature protein.
1) Fallah MJ et al., (2003) IJMS 28(3):131-134. (General)
2) Panopoulos AD and Watowich SS, (2008) Cytokine 42:277-288. (General)
3) Hirbe AC et al., (2007) Blood 109(8):3424-3431. (General)