G-CSF (human) (rec.)
|Synonyms||Granulocyte Colony-stimulating Factor; Pluripoietin|
|Sequence||Human G-CSF (aa30-207).|
|Biological Activity||The ED50 of G-CSF is typically 10-40pg/ml. measured in a cell proliferation assay using a murine myeloblastic M-NFS-60 cell line|
|Endotoxin Content||<1EU/µg purified protein (LAL test; Lonza).|
|Formulation||Liquid. In PBS, 10% glycerol.|
|Other Product Data||
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|Declaration||Manufactured by AbFrontier.|
|Shipping and Handling|
|Short Term Storage||-20°C|
|Long Term Storage||-80°C|
|Handling Advice||Avoid freeze/thaw cycles.|
|Product Specification Sheet|
Granulocyte colony-stimulating factor(G-CSF) is a hematopoietic cytokine that promotes the proliferation and differentiation of cells in the granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cells can also secrete G-CSF after IL-1b,TNF-alpha and LPS activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. G-CSF has a effect in the treatment of leukopenia, AIDS, MDS and bone marrow transplantation. In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid precursor proteins, have been isolated. Both protein have identical amino acid sequences except for a three amino acid insertion (deletion) at the 35th amino acid residue from the N-terminus of the mature protein.
1) Fallah MJ et al., 2003, IJMS 28(3):131-134. 2) Panopoulos AD and Watowich SS, 2008, Cytokine 42:277-288. 3) Hirbe AC et al., 2007, Blood 109(8):3424-3431.