ICAD (human) (rec.)

CHF 358.00
In stock
YIF-LF-P03310.2 mgCHF 358.00
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Product Details
Synonyms H13; DFFA; DFF1; ICAD; DFF-45; Inhibitor of CAD; DNA Fragmentation Factor Subunit α; DNA Fragmentation Factor 45 kDa Subunit
Product Type Protein
Source/Host E. coli
Crossreactivity Human
Biological Activity

Activity has not been tested.

Purity ≥95% (SDS-PAGE)
Formulation Lyophilized from 50mM NaH2PO4, pH8.0, 300mM NaCl, 130mM imidazole.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

Declaration Manufactured by AbFrontier
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Use/Stability After reconstitution, store at -80°C.
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF

The inhibitor of caspase-3-activated DNase (ICAD) is a caspase 3 substrate that controls nuclear apoptosis. ICAD has two isoforms: a functional isoform of 45kDa, ICAD-L/DNA fragmentation factor (DFF) 45; and a 35kDa isoform, ICAD-S/DFF35. Although both ICAD-L and ICAD-S can bind and inhibit CAD, only ICAD-L was reported to be functional. ICAD is cleaved to be inactivated and allow caspase-activated DNase (CAD) to execute nuclear internucleosomal apoptotic DNA fragmentation. In non-apoptotic cells, CAD is complexed with its inhibitor, ICAD. The activation of the CAD/ICAD complex occurs through the caspase 3-mediated cleavage of ICAD at residues 117 and 224, which results in three ICAD fragments that are then released from CAD. In addition to its DNase inhibitory activity, ICAD acts as a CAD specific folding chaperone. There are recent reports that ICAD is a potential target for restoring a normal apoptotic signal transduction pathway in colon and brain cancer cells.

Product References

1) Enari M et al., (1998) Nature 391(6662):43-50. (General)
2) Sakahira H et al., (1999) J Biol Chem 274(22):15740-4. (General)
3) Charrier L et al., (2002) Cancer Res 62(7):2169-74. (General)
4) Fukushima K et al., (2002) J Mol Biol 321(2):317-27. (General)

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