anti-GFP, pAb

CHF 322.00
In stock
YIF-LF-PA0043100 µlCHF 322.00
More Information
Product Details
Synonyms GFP; Green Fluorescent Protein
Product Type Polyclonal Antibody
Properties
Immunogen/Antigen Recombinant green fluorescent protein.
Application

ELISA
Western Blot (1:1,000~5,000)
Immunoprecipitation (1~2 μl)

Crossreactivity Human
Formulation Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

Declaration Manufactured by AbFrontier
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS No
Product Specification Sheet
Datasheet Download PDF

Green fluorescent protein (GFP) isolated from jellyfish Aequorea aequorea is a 238 amino acid protein with an apparent molecular weight of about 27-30 kDa on SDS-PAGE. Its chromophore is formed by cyclisation and oxidation of the three amino acids Ser65, Tyr66, and Gly67. The numerous applications include : using GFP as a reporter for gene expression, as a marker to study cell lineage during development and as a tag to localize proteins in living cells. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technology has considerably contributed to a greater understanding of cellular physiology. Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin.

Product References

1) Hans-Hermann Gerdes et al. (1996) FEBS Letters 389:44-47. (General)
2) J.C. March et al. (2003) Appl Microbiol Biotechnol 62:303-315. (General)

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