anti-PKC ε, pAb

CHF 322.00
In stock
YIF-LF-PA0084100 µlCHF 322.00
More Information
Product Details
Synonyms Pkce; Prkce; nPKC-ε; EC=2.7.11.13; Protein Kinase C ε Type
Product Type Polyclonal Antibody
Properties
Immunogen/Antigen Synthetic peptide.
Application

Western Blot (1:2,000)

Crossreactivity Mouse
Rat
Purity Detail Affinity purification.
Formulation Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

Declaration Manufactured by AbFrontier
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS No
Product Specification Sheet
Datasheet Download PDF

Protein kinase C (PKC) is a family of serine-threonine kinases that regulate a broad spectrum of cellular functions such as cell migration and polarity, proliferation, differentiation, and cell death. The family is composed of several genes that express structurally related phospholipid-dependent kinases with distinct means of regulation and tissue distribution. Based on their structures and sensitivities to Ca2+ and diacylglycerol (DAG), they have been classified into conventional PKCs (α, β, and γ), novel PKCs (δ, ε, η, and θ), and atypical PKCs (ζ and λ/ι). PKCs share a structural backbone, mainly consisting of a regulatory domain at the N-terminus and a catalytic domain at the C-terminus. All family members require phosphatidylserine, a component of the phospholipid bilayer, for their activation. Some PKCs (PKCα, δ, and ζ) are widely expressed in all tissues, but other isoforms are expressed in a tissue-specific manner. PKCγ, for example, is largely confined to brain and neuronal tissue, PKCι is mainly expressed in testis and insulin secreting cells, and PKCθ is mainly expressed in skeletal muscle. PKC ε (PKCε) is a calcium-independent and phorbolester/diacylglycerol-sensitive serine/threonine kinase. PKCε is the only PKC isozyme that has been shown to behave as an oncoprotein. Constitutive activation of PKCε in a small cell lung cancer (SCLC) cell line and the overexpression of PKCε in colonic epithelial cells have been reported. This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

Product References

1) Breitkreutz D. et al., (2007) J Cancer Res Clin Oncol. 133:793-808. (General)
2) Basu A. and Sivaprasad U., (2007) Cell Sinal. 19:1633-1642. (General)
3) Akita Y., (2002) J Biochem (Tokyo) 132:847-852. (General)
4) Nishizuka Y., (1995) FASEB J. 9:484-496. (General)

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