anti-β-Catenin (C-term), pAb

CHF 322.00
In stock
YIF-LF-PA0162100 µlCHF 322.00
 
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Product Details
Synonyms CTNNB; CTNNB1; β-Catenin; Catenin β-1
Product Type Polyclonal Antibody
Properties
Immunogen/Antigen Recombinant human His-β-Catenin (C-terminal fragment) protein purified from E. coli.
Application Western Blot (1:2,000)
Immunoprecipitation (1~2 μl)
Crossreactivity Human
Mouse
Rat
Formulation Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.
Other Product Data Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.
Declaration Manufactured by AbFrontier
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS No
Product Specification Sheet
Datasheet Download PDF
β-Catenin was a cytosolic protein originally identified through its association with the cadherin class of cell-adhesion proteins. β-Catenin has two key cellular functions; one plays direct role in cell adhesion/migration, bridging between cadherins and tactin cytoskeleton. The other plays as a transcription cofactor with T cell factor/lymphoid enhancer factor (TCF/LEF) in the Wnt pathway. Wnt are powerful regulators of cell proliferation and differentiation, and their signaling pathway involves proteins that directly participate in both gene transcription and cell adhesion. Activation of Wnt signaling leads to inhibition of Glycogen synthase-3β (GSK-3β) activity, resulting in accumulation of cytoplasmic (signaling) β-Catenin, which becomes available to bind the TCF/LEF family of transcription factors and to induce target gene expression. In the absence of Wnt signaling, A complex of axin and casein kinase-I(CK-I) induces β-Catenin phospho- rylation at a single site: serine 45(Ser45). This likely serves as a priming site for subsequent phosphorylation by GSK3β. And then β-Catenin is phosphorylated by GSK-3β at Ser33/37 and Thr41. These serine/threonine phosphylation is required for the phosph rylation-dependant degradation of β catenin via ubiquitin-proteosome pathway. Mutation of these phosphorylation sites in β-Catenin has been found in many tumor cell lines. Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.
Product References
1) Hinck L, et al. (1994)Trends Biochem Sci. 19(12):538-42. (General)
2) Schneider SQ, et al. (2003) J Exp Zoolog B Mol Dev Evol. 295(1):25-44. (General)
3) W. James Nelson et al. (2004) Science 303 :1483-7. (General)
4) Amit S, et al. (2002) Genes Dev. 16(9):1066-76. (General)
(5) Morin P.J., et al. (1997) Science 275:1787-1790. (General)
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