Cookie Policy: This site uses cookies to improve your experience. You can find out more about our use of cookies in our Privacy Policy. By continuing to browse this site you agree to our use of cookies.
anti-PKC α (Protein Kinase C α), pAb
Product Details | |
---|---|
Synonyms | PKCA; PKC-A; PRKCA; PKC-α; EC=2.7.11.13; Protein Kinase C α Type |
Product Type | Polyclonal Antibody |
Properties | |
Immunogen/Antigen | Synthetic peptide. |
Application |
Western Blot (1:2,000) |
Crossreactivity |
Human Mouse Rat |
Purity Detail | Affinity purification. |
Formulation | Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. |
Other Product Data |
Click here for Original Manufacturer Product Datasheet |
Declaration | Manufactured by AbFrontier |
Shipping and Handling | |
Shipping | BLUE ICE |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
Documents | |
MSDS | Inquire |
Product Specification Sheet | |
Datasheet |
![]() |
Protein kinase C (PKC) is a family of serine-threonine kinases that regulate a broad spectrum of cellular functions. The family is composed of nine genes that express structurally related phospholipid-dependent kinases with distinct means of regulation and tissue distribution. Based on their structures and sensitivities to Ca2+ and diacylglycerol (DAG), they have been classified into conventional PKCs (α, β, and γ), novel PKCs (δ, ε, η, and θ), and atypical PKCs (ζ and λ/ι). Mammalian PKC α consists of 672 amino acids and is distributed in all tissues, in contrast to other PKC isotypes whose expression is restricted in the particular tissues. PKC α is activated by a variety of stimuli originating from receptor activation, cell contact and physical stresses. Kinase activity of PKC α is regulated by phosphorylation of three conserved residues in its kinase domain: the activation-loop site Thr-497, the autophosphorylation site Thr-638, and the hydrophobic C-terminal site Ser-657. In some types of cells, PKC α is implicated in cell growth, in contrast, it may play a role in cell cycle arrest and differentiation in other types of cells. The responses are modulated by dynamic interactions with cell-type specific factors. This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
1) Nakashima, S., (2002) J Biochem (Tokyo). 132:669-675. (General)
2) Saito N. and Shirai Y., (2002) J Biochem (Tokyo). 132:683-687. (General)
3) Hansra, G. et al., (1999) Biochem. J. 342:337-344. (General)