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anti-GAPDH, pAb
Product Details | |
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Synonyms | GAPD; GAPDH; CDABP0047; EC=2.6.99.-; EC=1.2.1.12; Peptidyl-Cysteine S-Nitrosylase GAPDH; Glyceraldehyde-3-Phosphate Dehydrogenase; Loading Control |
Product Type | Polyclonal Antibody |
Properties | |
Immunogen/Antigen | Recombinant human protein purified from E. coli. |
Application |
Western Blot (1:10,000) |
Crossreactivity |
Human Mouse Rat |
Purity Detail | Protein A purified. |
Concentration | 0.2 mg/ml |
Formulation | Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. |
Other Product Data |
Click here for Original Manufacturer Product Datasheet |
Declaration | Manufactured by AbFrontier |
Shipping and Handling | |
Shipping | BLUE ICE |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
Documents | |
MSDS | Inquire |
Product Specification Sheet | |
Datasheet |
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Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a catalytic enzyme commonly known to be involved in glycolysis. The enzyme exists as a tetramer of identical 37 kDa subunits. GAPDH catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, this ubiquitously expressed enzyme also displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication (1), DNA repair (2), nuclear RNA export (3-4), membrane fusion (5) and microtubule bundling. Other studies also provide evidence of GAPDH playing an essential part of the program of gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases (6-7). Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
1) Baxi, M. D. and Vishwanatha, J. K. (1995) Biochemistry. 34, 9700-9707. (General)
2) McNulty, S. E. and Toscano, W. A. Jr. (1995) Biochem. Biophys. Res. Commun. 34, 165-171. (General)
3) Singh, R. and Green, M. R. (1993) Science. 259, 365-368. (General)
4) Zang, W. Q. et al. (1998) Virology. 28, 46-52. (General)
5) Han, X. et al. (1998) Biochem. Biophys. Acta. 1414, 95-107. (General)
6) Kragten, E. et al. (1998) J. Biol. Chem. 273, 5821-5828. (General)
7) Koshy, B. (1996) Hum. Mol. Genet. 5, 1311-1318. (General)