HeLa Cell Lysate (human)

CHF 52.00
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YIF-LF-R0001200 µlCHF 52.00
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Product Details
Product Type Protein
Source/Host HeLa cells
Crossreactivity Human
Application Western Blot
Concentration 1mg/ml
Other Product Data Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.
Boil for 5minutes to reduce the preparation. Load 20ug of reduced lysate per lane for Immunoblot analysis.
Declaration Manufactured by AbFrontier
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Product Specification Sheet
Datasheet Download PDF
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate. Cells were lysed in Lysis buffer (20mM HEPES, pH7.2, 20mM Phosphoglycerate, 150mM NaCl, 10% glycerol, 1% NP-40, 1mM EDTA, 1mM EGTA, 1mM Na3VO4, 5mM NaF, 1mM PMSF, 5ug/ml Pepstatin A, 5ug/ml Aprotinin, 5ug/ml Leupeptin) and diluted with reducing sample buffer (60mM Tris, pH 6.8, 6% glycerol, 2% SDS, 0.2% bromphenol blue, 200mM 2-mercaptoethanol).
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