HeLa Cell Lysate (human) (H2O2 stimulated)
CHF 102.00
In stock
YIF-LF-R00021 SetCHF 102.00
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| Product Details | |
|---|---|
| Product Type | Protein |
| Properties | |
| Source/Host | HeLa cells |
| Crossreactivity | Human |
| Application |
Western Blot |
| Concentration | 1mg/ml |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| Declaration | Manufactured by AbFrontier |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 6 months after receipt when stored at -20°C. |
| Documents | |
| MSDS | No |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Cells were cultured for 30 minutes in the presence of 1mM H2O2 (only LF-R0002A - H O stimulated HeLa cell lysate). Then cells were lysed in Lysis buffer (20mM HEPES, pH7.2, 20mM Phosphoglycerate, 150mM NaCl, 10% glycerol, 1% NP-40, 1mM EDTA, 1mM EGTA, 1mM Na3VO4, 5mM NaF, 1mM PMSF, 5ug/ml Pepstatin A, 5ug/ml Aprotinin, 5ug/ml Leupeptin) and diluted with reducing sample buffer (60mM Tris, pH 6.8, 6% glycerol, 2% SDS, 0.2% bromphenolblue, 200mM 2-mercaptoethanol).
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