HeLa Cell Lysate (human) (H2O2 stimulated)
YIF-LF-R00021 SetCHF 102.00
|Other Product Data||
Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.
Boil for 5minutes to reduce the preparation. Load 20ug of reduced lysate per lane for Immunoblot analysis.
|Declaration||Manufactured by AbFrontier|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
|Handling Advice||Avoid freeze/thaw cycles.|
|Use/Stability||Stable for at least 6 months after receipt when stored at -20°C.|
|Product Specification Sheet|
Cells were cultured for 30 minutes in the presence of 1mM H2O2 (only LF-R0002A - H O stimulated HeLa cell lysate). Then cells were lysed in Lysis buffer (20mM HEPES, pH7.2, 20mM Phosphoglycerate, 150mM NaCl, 10% glycerol, 1% NP-40, 1mM EDTA, 1mM EGTA, 1mM Na3VO4, 5mM NaF, 1mM PMSF, 5ug/ml Pepstatin A, 5ug/ml Aprotinin, 5ug/ml Leupeptin) and diluted with reducing sample buffer (60mM Tris, pH 6.8, 6% glycerol, 2% SDS, 0.2% bromphenolblue, 200mM 2-mercaptoethanol).