Jurkat Cell Lysate (human)
|Other Product Data||
Click here for Original Manufacturer Product Datasheet
|Declaration||Manufactured by AbFrontier|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
Avoid freeze/thaw cycles.
For maximum product recovery after thawing, centrifuge the vial before opening the cap.
|Use/Stability||Stable for at least 6 months after receipt when stored at -20°C.|
|Product Specification Sheet|
Jurkat (Human T-lymphocyte cell line) Whole Cell Lysate. Cells were lysed in Lysis buffer (20mM HEPES, pH7.2, 20mM Phosphoglycerate, 150mM NaCl, 10% glycerol, 1% NP-40, 1mM EDTA, 1mM EGTA, 1mM Na3VO4, 5mM NaF, 1mM PMSF, 5ug/ml Pepstatin A, 5ug/ml Aprotinin, 5ug/ml Leupeptin) and diluted with reducing sample buffer (60mM Tris, pH 6.8, 6% glycerol, 2% SDS, 0.2% bromphenol blue, 200mM 2- mercaptoethanol).