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AdipoGen Life Sciences
anti-LTβR (mouse), mAb (3C8)
370
CHF
CHF 370.00
In stock
AG-20B-0041-C100100 µgCHF 370.00
| Product Details | |
|---|---|
| Synonyms | Lymphotoxin-β Receptor; Tumor Necrosis Factor Receptor 2 Related Protein; Tumor Necrosis Factor C Receptor; Tumor Necrosis Factor Receptor Superfamily Member 3; TNFRSF3 |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | 3C8 |
| Isotype | Rat IgG1κ |
| Source/Host | Purified from concentrated hybridoma tissue culture supernatant. |
| Immunogen/Antigen | Recombinant mouse LTβR (cysteine-rich region/aa 31-221). |
| Application |
Functional Application: Agonist inducing BAFF, chemokines and integrins in vitro and in vivo. |
| Crossreactivity | Mouse |
| Specificity |
Recognizes mouse LTβR. |
| Purity | ≥95% (SDS-PAGE) |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. |
| Isotype Negative Control | |
| Other Product Data |
The monoclonal antibody to mouse LTβR is an agonist that can be used for the investigation of the regulation of BAFF (BlyS), chemokines and integrins using in vivo and tissue culture models, the development of NK cells and NK T cells, to study the regulation of NF-κB family of transcription factors in regulation of inflammation and homeostasis, particularly RelB NF-κB2 pathway. For use as an agonist the MAb to LTβR is added to cell cultures at 2μg/ml. For in vivo use, mice are injected intraperitoneally with 50μg of agonistic MAb to LTβR in sterile phosphate saline buffer. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The LTβR activates two different NF-κB pathways that lead to distinct patterns of gene induction, including selected chemokines and the cytokine BAFF, which is essential for the survival of mature B lymphocytes. LTβR activates the classical NF-κB (relA/p50) pathway, like the type 1 TNF receptor (TNFR1), that regulates proinflammatory genes, like the chemokine MIP1β. However, LTβR, unlike TNFR1, also activates the processing of p100 to form RelB/p52 complexes, which activate genes involved in lymphoid organ formation and lymphocyte survival.
Product References
- Differential regulation of CCL21 in lymphoid/nonlymphoid tissues for effectively attracting T cells to peripheral tissues: J.C. Lo, et al.; J. Clin. Invest. 112, 1495 (2003)
- A lymphotoxin-IFN-beta axis essential for lymphocyte survival revealed during cytomegalovirus infection: T.A. Banks, et al.; J. Immunol. 174, 7217 (2005)
- Specific Remodeling of Splenic Architecture by Cytomegalovirus: C.A. Benedict et al.; PLoS Pathog. 2, e16 (2006)
- A fourth IkappaB protein within the NF-kappaB signaling module: S. Basak, et al.; Cell 128, 369 (2007)
- A lymphotoxin-driven pathway to hepatocellular carcinoma: J. Haybaeck, et al.; Cancer Cell 16, 295 (2009)
- LTβR Signaling Induces Cytokine Expression and Up-Regulates Lymphangiogenic Factors in Lymph Node Anlagen: M.F. Vondenhoff, et al.; J. Immunol. 182, 5439 (2009)
- Lymphotoxin Controls the IL-22 Protection Pathway in Gut Innate Lymphoid Cells during Mucosal Pathogen Challenge: A.V. Tumanov, et al.; Cell Host Microbe 10, 44 (2011)
- Multiple roles of lymphatic vessels in peripheral lymph node development: E. Bovay, et al.; J. Exp. Med. 215, 2760 (2018)
- Phosphatase PTPN22 regulates dendritic cell homeostasis and cDC2 dependent T cell responses: H. A. Purvis, et al.; Front. Immunol. 11, 376 (2020)
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