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AdipoGen Life Sciences
anti-Caspase-2, mAb (11B4)
450
CHF
CHF 450.00
In stock
AG-20T-0136-C100100 µgCHF 450.00
Figure 1: Western blot analysis using anti-caspase-2, mAb (11B4) (Prod. No. AG-20T-0136), detecting caspase-2 expression in cultured mouse and human cell lines. Caspase-2 protein was revealed in lysates from 2x105 mouse and human cell lines by 11B4 and ECL.
Figure 2: Immunoprecipitation using anti-caspase-2, mAb (11B4) (Prod. No. AG-20T-0136), an isotype-matched control antibody or an anti-HA mAb of 35S-labelled 293T cells transfected with mutant caspase-2-HA.
| Product Details | |
|---|---|
| Synonyms | CASP-2; Protease ICH-1; Neural Precursor Cell Expressed Developmentally Down-regulated Protein 2; NEDD-2 |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | 11B4 |
| Isotype | Rat IgG2aκ |
| Immunogen/Antigen | Recombinant human caspase-2 (p19 subunit). |
| Application |
Immunoprecipitation: (1-5μg/ml) |
| Crossreactivity |
Dog Human Monkey Mouse Rat |
| Specificity |
Recognizes an epitope in the p19 subunit of human, mouse, rat, monkey and dog caspase-2. Detects bands of ~51kDa (procaspase-2), ~32kDa and ~18kDa (cleavage products) by Western blot. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified (sepharose column). |
| Endotoxin Content | <0.1EU/μg purified protein (LAL test; Lonza). |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 0.02% sodium azide. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Caspase-2 is a Class I caspase with a long prodomain necessary for nuclear localization. Upon activation of the apoptotic pathway, the procaspase is cleaved into smaller fragments. Caspase-2 is the nuclear apoptotic respondent to cellular genotoxic stress or mitotic catastrophe and is involved in the activation cascade of caspases responsible for apoptosis execution. Activation occurs upon recruitment to a complex containing a p53-induced death domain protein, PIDD. This suggests caspase-2 can be a nuclear initiator caspase with a requirement for caspase-9 and caspase-3 activation in downstream apoptotic events. In apoptotic pathways resulting from UV-induced DNA damage, processing of caspase-2 occurs downstream of mitochondrial dysfunction and of caspase-9 and caspase-3 activation, extending a possible role for caspase-2 as a parallel effector caspase.
Product References
- Caspase-2 is not required for thymocyte or neuronal apoptosis even though cleavage of caspase-2 is dependent on both Apaf-1 and caspase-9: L.A. O'Reilly, et al.; Cell Death Differ. 9, 832 (2002)
- A novel Apaf-1–independent putative caspase-2 activation complex: S.H. Read, et al.; J. Cell Biol. 159, 739 (2002)
- A Novel Caspase-2 Complex Containing TRAF2 and RIP1: M. Lamkanfi, et al.; J. Biol. Chem. 280, 6923 (2005)
- Death domain assembly mechanism revealed by crystal structure of the oligomeric PIDDosome core complex: H.H. Park, et al.; Cell 128, 533 (2007)
- Caspase-2 activation in the absence of PIDDosome formation: C. Manzl, et al.; J. Cell Biol. 185, 291 (2009)
- LBH589, a deacetylase inhibitor, induces apoptosis in adult T-cell leukemia/lymphoma cells via activation of a novel RAIDD-caspase-2 pathway: H. Hasegawa, et al.; Leukemia 25, 575 (2011)
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