AdipoGen Life Sciences

anti-SENP2 (mouse), pAb

CHF 190.00

In stock

AG-25A-0078-C100100 µgCHF 190.00

Figure 1: Western blot analysis using anti-SENP2 (mouse), pAb (Prod. No. AG-25A-0078) at 1:2'000 dilution.
1: Mouse SENP2 (catalytic domain) (His-tagged).
2: Mouse brain cell lysate (Balb/c mouse, 70μg)
3: Mouse IDO (His-tagged) (negative control).

Specifications / Handling

More Information
Product Details
Synonyms	Axam2; Smt3ip2; Sentrin-specific Protease 2
Product Type	Polyclonal Antibody
Properties
Source/Host	Rabbit
Immunogen/Antigen	Recombinant mouse SENP2 (catalytic domain).
Application	ELISA: (direct and indirect: 1:2'000-1:5'000) Western Blot: (1:2’000-1:5’000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application. Note: Tested on recombinant proteins and/or target-protein transfected cell lines in ELISA, Western Blot and/or FACS.
Crossreactivity	Mouse
Specificity	Recognizes mouse SENP2. Detects a band of ~26kDa (rec) and ~68kDa in mouse brain lysate by Western blot.
Purity Detail	Protein A-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Rabbit IgG
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

SENP2 is a protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. Downregulates CTNNB1 levels and thereby modulates the Wnt pathway.

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