VISTA (human):Fc (human) (recombinant protein)

Specifications / Handling

More Information
Product Details
Synonyms	V-type Immunoglobulin Domain-containing Suppressor of T Cell Activation; Platelet Receptor Gi24; Stress-induced Secreted Protein-1; Sisp-1; V-set Domain-containing Immunoregulatory Receptor
Product Type	Protein
Properties
Source/Host	HEK 293 cells
Sequence	The extracellular domain of human VISTA (aa 33-194) is fused at the C-terminus to the Fc portion of human IgG1.
Crossreactivity	Human Mouse
MW	~65-70kDa (SDS-PAGE)
Purity	≥95% (SDS-PAGE)
Endotoxin Content	<0.01EU/μg purified protein (LAL test).
Concentration	1mg/ml after reconstitution.
Reconstitution	Reconstitute with 50μl sterile water.
Formulation	Lyophilized. Contains PBS.
Protein Negative Control	Fc (human) IgG1 Control (rec.)
Other Product Data	UniProt link Q9H7M9: VISTA (human)
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Human VISTA is a 55- to 65kDa type I Ig membrane protein with the extracellular domain homologous to PD-L1. VISTA is mainly expressed on hematopoietic tissues (spleen, thymus and bone marrow) and on myeloid cells with lower expression on CD4+ and CD8+ T cells. VISTA is a new negative checkpoint regulator that potently suppresses T cell activation. Overexpression of VISTA on tumors inhibits the protective anti-tumor immunity and blockade of VISTA enhances antitumor immunity in multiple tumor models. VISTA on APC delivers negative signaling to T cells via a yet-to-identify binding partner on T cells.

Call	+41 61 926 6040
Fax	+41 61 926 6049

VISTA [B7-H5] (human):Fc (human) (rec.)