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AdipoGen Life Sciences
PARG (human) (rec.) (His) (highly active)
290
CHF
CHF 290.00
In stock
AG-40T-0022-C0022 µgCHF 290.00
| Product Details | |
|---|---|
| Synonyms | Poly(ADP-ribose) Glycohydrolase |
| Product Type | Protein |
| Properties | |
| Source/Host | Sf21 cells |
| Sequence | Human PARG is fused to a His-tag. |
| Crossreactivity | Human |
| Biological Activity | ~100ng is required for poly(ADP-ribose) degradation assays. |
| Purity | ≥95% (SDS-PAGE) |
| Concentration | Lot dependent (0.1-1.5mg/ml) |
| Formulation | Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 0.2% NP-40, 50mM imidazole and 10% glycerol. |
| Other Product Data |
UniProt ID Q86W56: PARG (human) |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -20°C |
| Long Term Storage | -80°C |
| Handling Advice |
After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 6 months after receipt when stored at -80°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by poly(ADP-ribose) glycohydrolase. PARG hydrolyzes poly(ADP-ribose) at glycosidic (1''-2') linkage of ribose-ribose bond to produce free ADP-ribose.
Product References
- Macrodomain-containing proteins are new mono-ADP-ribosylhydrolases: F. Rosenthal, et al.; Nat. Struct. Mol. Biol. 20, 502 (2013)
- Liquid demixing of intrinsically disordered proteins is seeded by poly(ADP-ribose): M. Altmeyer, et al.; Nat. Commun. 6, 8088 (2015)
- 5-diphosphoribose is an endogenous TRPM2 superagonist: R. Fliegert, et al.; Nat. Chem. Biol. 13, 1036 (2017)
- PARP1 catalytic variants reveal branching and chain length-specific functions of poly(ADP-ribose) in cellular physiology and stress response: L. Aberle, et al.; Nucleic Acid Res. 48, 10015 (2020)
- The Ubiquitin Ligase TRIP12 Limits PARP1 Trapping and Constrains PARP Inhibitor Efficiency: M. Gatti, et al.; Cell Rep. 32, 107985 (2020)
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