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Chemodex
BCECF-AM
197
CHF
CHF 197.00
In stock
CDX-C0003-M0011 mgCHF 197.00
CDX-C0003-M0055 mgCHF 788.00
| Product Details | |
|---|---|
| Synonyms | 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester; Spiro(isobenzofuran-1(3H),9'-(9H)xanthene)-2',7'-dipropanoic acid |
| Product Type | Chemical |
| Properties | |
| Formula |
C42H40O21 |
| MW | 808.69 |
| CAS | 117464-70-7 |
| Source/Host Chemicals | Synthetic. |
| Purity Chemicals | ≥95% (HPCE) |
| Appearance | Red semi-solid. |
| Solubility | Soluble in DMSO, DMF or acetonitrile. |
| Identity | Determined by 1H-NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | NTECHUXHORNEGZ-UHFFFAOYSA-N |
| Smiles | CC(=O)OCOC(=O)CCC1=CC2=C(OC3=CC(OCOC(C)=O)=C(CCC(=O)OCOC(C)=O)C=C3C22OC(=O)C3=C2C=CC(=C3)C(=O)OCOC(C)=O)C=C1OCOC(C)=O |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Widely-used fluorescent indicator for estimating intracellular pH. This membrane-permeant compound is modified by intracellular esterases to produce BCECF, a polar fluorescein derivative that is retained by cells. Intracellular BCECF is stable and has an efflux half-life of greater than two hours. Intracellular pH measurements are made by ratio imaging, which involves determining the pH-dependent ratio of emission intensity (detected at 535nm) when the dye is excited at 490nm vs. the emission intensity when excited at 440nm. This approach is amenable to either spectrofluorometry or flow cytometry. BCECF-AM ester can also be used to investigate intracellular changes in other ions, including potassium. Spectral properties: λex 490nm; λem 535nm; λex=505nm; λem=520nm in DMSO.
Product References
(1) T.J. Rink, et al.; J. Cell Biol. 95, 189 (1982) | (2) C. Dive, et al.; Mol. Cell Probe 2, 131 (1988) | (3) A.K. Ho, et al.; Am. J. Physiol. 261, C642 (1991) | (4) W. Obexer, et al; Tropical Med. Parasit. 46(1), 45 (1995) | (5) S. Chow, et al.; Cytometry 24, 360 (1996) | (6) H. Urano, et al.; Chem. Lett. 12, 1217 (1997) | (7) L. Balkay, et al.; Cytometry 28, 42 (1997) | (8) P. Ozkan & R. Mutharasan; Biochim. Biophys Acta 1572, 143 (2002) | (9) N. O'Connor & R.B. Silver; Meth. Cell Biol. 81, 415 (2007)
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